Weiss L, Ward P M, Harlos J P
Int J Cancer. 1986 Aug 15;38(2):207-13. doi: 10.1002/ijc.2910380210.
The surface charge densities of Lewis lung carcinoma (3LL) cells were measured in tumors transplanted into subcutaneous (s.c.) sites, and into the kidneys (K), as well as in kidney tumors transplanted to, and maintained in, the s.c. site (K.s.c.). K cells showed a significant increase in net negativity in comparison with s.c. cells, which was stable over II passages. On back-transplantation of K cells to the s.c. site, the net negativity of K.s.c. cells was higher than that of the s.c. cells, but the increase was stable for only 7 passages; on subsequent passages the net negativity fell, approaching that of the s.c. cells. Detailed analyses suggest the presence in these tumors of 2 subpopulations of 3LL cells, whose proportions change in the different sites studied to produce changes in the mean electrophoretic mobilities of the whole cancer cell populations. The altered proportions of the 2 cell types in the 3 sites were not associated with major changes either in their general behavior with respect to metastasis, or in differential growth in various organs following s.c., tail vein, and left ventricular injections. Selection induced by growth in the kidney, as evidenced by physiochemical changes at the cancer cell surface, was not associated with kidney preference in metastasis or metastasis-related behavior.
在移植到皮下（s.c.）部位、肾脏（K）以及移植到皮下部位并维持生长的肾肿瘤（K.s.c.）中的Lewis肺癌（3LL）细胞的表面电荷密度进行了测量。与皮下细胞相比，K细胞的净负电荷显著增加，且在11代传代过程中保持稳定。将K细胞回植到皮下部位时，K.s.c.细胞的净负电荷高于皮下细胞，但这种增加仅在7代传代中保持稳定；在随后的传代中，净负电荷下降，接近皮下细胞的水平。详细分析表明，这些肿瘤中存在两个3LL细胞亚群，在不同研究部位其比例发生变化，从而导致整个癌细胞群体的平均电泳迁移率发生改变。这两种细胞类型在三个部位的比例变化，与其转移的总体行为或皮下、尾静脉和左心室注射后在各个器官中的差异生长的主要变化无关。由在肾脏中生长所诱导的选择，如癌细胞表面的物理化学变化所证明的，与转移中的肾脏偏好或转移相关行为无关。
