Hossain A, Sarkar N H
Department of Immunology and Microbiology, Medical College of Georgia, Augusta, 30912-2100.
Clin Exp Metastasis. 1991 Jul-Aug;9(4):351-61. doi: 10.1007/BF01769355.
Metastasis by mouse mammary tumor cells is usually confined to lung. This paper describes the metastatic behavior of an established mouse mammary tumor cell line, 4526, that in addition to lung and liver metastasis, shows a high rate of heart metastases. The tumor cells were inoculated into the fourth mammary fat pad of syngeneic mice and their pattern of distant colonization was analysed qualitatively as well as quantitatively. We found that the cell line produced 100, 70 and 40% metastases to the lung, liver and heart, respectively. While the lung metastases appeared primarily as nodular masses, the liver metastases occurred both as nodular and diffuse masses. In addition, we observed that the metastatic load of each of the different lung lobes of individual mice was proportional directly to its relative size, and there seemed to be an inverse relationship between the occurrence of lung and liver metastases in individual mice. As compared to lung and liver metastases, heart metastases were found to be localized internally, usually in the cavity and wall of the ventricle. Furthermore, hearts with metastases revealed destruction of cardiac tissue and blockage of the cavity space. Our results show that 4526 cells are phenotypically stable, since the metastatic behavior of several clonal derivatives of the cell line obtained from lung, liver and heart colonies were found to be identical to that of the parental cell line. Thus this cell line, because of its unparalleled metastatic characteristics, offers a model for investigations into the biology of mammary tumor cell metastasis, especially heart metastasis.
小鼠乳腺肿瘤细胞的转移通常局限于肺部。本文描述了一种已建立的小鼠乳腺肿瘤细胞系4526的转移行为，该细胞系除了能转移至肺和肝脏外，还表现出较高的心脏转移率。将肿瘤细胞接种到同基因小鼠的第四乳腺脂肪垫中，并对其远处定植模式进行定性和定量分析。我们发现该细胞系分别有100%、70%和40%的细胞转移至肺、肝脏和心脏。肺转移主要表现为结节状肿块，而肝转移则既有结节状也有弥漫性肿块。此外，我们观察到个体小鼠不同肺叶的转移负荷与其相对大小直接成正比，并且个体小鼠肺转移和肝转移的发生之间似乎存在反比关系。与肺和肝转移相比，心脏转移位于内部，通常在心室腔和心室壁。此外，有转移的心脏显示出心脏组织破坏和腔隙阻塞。我们的结果表明4526细胞表型稳定，因为从肺、肝脏和心脏集落获得的该细胞系的几个克隆衍生物的转移行为与亲代细胞系相同。因此，由于其无与伦比的转移特性，该细胞系为研究乳腺肿瘤细胞转移生物学，尤其是心脏转移，提供了一个模型。
