Pirona Raul, Frugis Giovanna, Locatelli Franca, Mattana Monica, Genga Annamaria, Baldoni Elena
National Research Council (CNR), Institute of Agricultural Biology and Biotechnology (IBBA), Milano, Italy.
National Research Council (CNR), Institute of Agricultural Biology and Biotechnology (IBBA), Rome Unit, Roma, Italy.
Front Plant Sci. 2023 Jun 2;14:1155797. doi: 10.3389/fpls.2023.1155797. eCollection 2023.
Tomato ( L.) is a major horticultural crop that is cultivated worldwide and is characteristic of the Mediterranean agricultural system. It represents a key component of the diet of billion people and an important source of vitamins and carotenoids. Tomato cultivation in open field often experiences drought episodes, leading to severe yield losses, since most modern cultivars are sensitive to water deficit. Water stress leads to changes in the expression of stress-responsive genes in different plant tissues, and transcriptomics can support the identification of genes and pathways regulating this response.
Here, we performed a transcriptomic analysis of two tomato genotypes, M82 and Tondo, in response to a PEG-mediated osmotic treatment. The analysis was conducted separately on leaves and roots to characterize the specific response of these two organs.
A total of 6,267 differentially expressed transcripts related to stress response was detected. The construction of gene co-expression networks defined the molecular pathways of the common and specific responses of leaf and root. The common response was characterized by ABA-dependent and ABA-independent signaling pathways, and by the interconnection between ABA and JA signaling. The root-specific response concerned genes involved in cell wall metabolism and remodeling, whereas the leaf-specific response was principally related to leaf senescence and ethylene signaling. The transcription factors representing the hubs of these regulatory networks were identified. Some of them have not yet been characterized and can represent novel candidates for tolerance.
This work shed new light on the regulatory networks occurring in tomato leaf and root under osmotic stress and set the base for an in-depth characterization of novel stress-related genes that may represent potential candidates for improving tolerance to abiotic stress in tomato.
番茄（L.）是一种主要的园艺作物，在全球范围内广泛种植，是地中海农业系统的典型代表。它是数十亿人饮食中的关键组成部分，也是维生素和类胡萝卜素的重要来源。由于大多数现代番茄品种对水分亏缺敏感，露地番茄种植经常遭遇干旱，导致严重的产量损失。水分胁迫会导致不同植物组织中应激反应基因的表达发生变化，转录组学可以帮助识别调控这种反应的基因和途径。
在此，我们对两种番茄基因型M82和Tondo进行了转录组分析，以响应聚乙二醇（PEG）介导的渗透处理。分析分别在叶片和根系上进行，以表征这两个器官的特定反应。
共检测到6267个与应激反应相关的差异表达转录本。基因共表达网络的构建确定了叶片和根系共同及特定反应的分子途径。共同反应的特征是依赖脱落酸（ABA）和不依赖ABA的信号通路，以及ABA和茉莉酸（JA）信号之间的相互联系。根系特异性反应涉及参与细胞壁代谢和重塑的基因，而叶片特异性反应主要与叶片衰老和乙烯信号有关。确定了代表这些调控网络核心的转录因子。其中一些尚未得到表征，可能是耐受性的新候选基因。
这项工作为渗透胁迫下番茄叶片和根系中发生的调控网络提供了新的见解，并为深入表征新的胁迫相关基因奠定了基础，这些基因可能是提高番茄对非生物胁迫耐受性的潜在候选基因。
