Jacob Valentine, Lambour Alexis, Swinyard Benjamin, Zerbib Yoann, Diouf Momar, Soudet Simon, Brochot Etienne, Six Isabelle, Maizel Julien, Slama Michel, Guillaume Nicolas
Department of Human Biology Center, Amiens University Medical Center, Amiens, France.
EA HEMATIM 4666, Jules Verne University of Picardie, Amiens, France.
Front Med (Lausanne). 2023 Jun 2;10:1186122. doi: 10.3389/fmed.2023.1186122. eCollection 2023.
To evaluate extracellular vesicles levels in a cohort of SARS-CoV-2's patients hospitalized in an intensive care unit with and without COVID-19 associated thromboembolic events.
In this study, we aim to assess endothelial and platelet membrane-derived extracellular vesicles levels in a cohort of SARS-CoV-2 patients with and without COVID-19-associated thromboembolic events who were hospitalized in an intensive care unit. Annexin-V positive extracellular vesicles levels were prospectively assessed by flow cytometry in one hundred twenty-three critically ill adults diagnosed with acute respiratory distress syndrome associated with a SARS-CoV-2 infection, ten adults diagnosed for moderate SARS-CoV-2 infection and 25 healthy volunteers.
On our critically ill patients, thirty-four patients (27.6%) had a thromboembolic event, Fifty-three (43%) died. Endothelial and platelet membrane-derived extracellular vesicles were drastically increased in SARS-CoV-2 patients hospitalized in the ICU compared to healthy volunteers. Moreover a slighty higher small/large ratio for platelets membrane-derived extracellular vesicles in patients was linked to thrombo-embolic events.
A comparison between total annexin-V positive extracellular vesicles levels in severe and moderate SARS-CoV-2 infection and healthy controls showed a significant increase in patients with severe infection and their sizes could be considered as biomarkers of SARS-CoV-2 associated thrombo-embolic events.
评估入住重症监护病房的一组感染严重急性呼吸综合征冠状病毒2（SARS-CoV-2）患者中，伴有和不伴有2019冠状病毒病（COVID-19）相关血栓栓塞事件时的细胞外囊泡水平。
在本研究中，我们旨在评估入住重症监护病房的一组伴有和不伴有COVID-19相关血栓栓塞事件的SARS-CoV-2患者中，内皮细胞和血小板膜来源的细胞外囊泡水平。通过流式细胞术对123例被诊断为与SARS-CoV-2感染相关的急性呼吸窘迫综合征的重症成年患者、10例被诊断为中度SARS-CoV-2感染的成年患者和25名健康志愿者，前瞻性地评估膜联蛋白V阳性细胞外囊泡水平。
在我们的重症患者中，34例患者（27.6%）发生了血栓栓塞事件，53例（43%）死亡。与健康志愿者相比，入住重症监护病房的SARS-CoV-2患者中，内皮细胞和血小板膜来源的细胞外囊泡显著增加。此外，患者中血小板膜来源的细胞外囊泡的小/大比率略高与血栓栓塞事件有关。
对重症和中度SARS-CoV-2感染患者及健康对照者的总膜联蛋白V阳性细胞外囊泡水平进行比较，结果显示重症感染患者的该水平显著升高，其大小可被视为SARS-CoV-2相关血栓栓塞事件的生物标志物。
