循环血小板衍生细胞外囊泡是新冠病毒感染的一个标志。
Circulating Platelet-Derived Extracellular Vesicles Are a Hallmark of Sars-Cov-2 Infection.
作者信息
Cappellano Giuseppe, Raineri Davide, Rolla Roberta, Giordano Mara, Puricelli Chiara, Vilardo Beatrice, Manfredi Marcello, Cantaluppi Vincenzo, Sainaghi Pier Paolo, Castello Luigi, De Vita Nello, Scotti Lorenza, Vaschetto Rosanna, Dianzani Umberto, Chiocchetti Annalisa
机构信息
Department of Health Sciences, Interdisciplinary Research Center of Autoimmune Diseases-IRCAD, Università del Piemonte Orientale, 28100 Novara, Italy.
Center for Translational Research on Autoimmune and Allergic Disease-CAAD, Università del Piemonte Orientale, 28100 Novara, Italy.
出版信息
Cells. 2021 Jan 7;10(1):85. doi: 10.3390/cells10010085.
Sars-Cov-2 infection causes fever and cough that may rapidly lead to acute respiratory distress syndrome (ARDS). Few biomarkers have been identified but, unfortunately, these are individually poorly specific, and novel biomarkers are needed to better predict patient outcome. The aim of this study was to evaluate the diagnostic performance of circulating platelets (PLT)-derived extracellular vesicles (EVs) as biomarkers for Sars-Cov-2 infection, by setting a rapid and reliable test on unmanipulated blood samples. PLT-EVs were quantified by flow cytometry on two independent cohorts of Sars-CoV-2+ (n = 69), Sars-Cov-2- (n = 62) hospitalized patients, and healthy controls. Diagnostic performance of PLT-EVs was evaluated by receiver operating characteristic (ROC) curve. PLT-EVs count were higher in Sars-Cov-2+ compared to Sars-Cov-2- patients or HC. ROC analysis of the combined cohorts showed an AUC = 0.79 and an optimal cut-off value of 1472 EVs/μL, with 75% sensitivity and 74% specificity. These data suggest that PLT-EVs might be an interesting biomarker deserving further investigations to test their predictive power.
严重急性呼吸综合征冠状病毒2（Sars-CoV-2）感染会引发发热和咳嗽，可能迅速导致急性呼吸窘迫综合征（ARDS）。目前已确定的生物标志物较少，但遗憾的是，这些标志物各自的特异性都很差，因此需要新的生物标志物来更好地预测患者的预后。本研究的目的是通过对未处理的血样进行快速可靠的检测，评估循环血小板（PLT）衍生的细胞外囊泡（EVs）作为Sars-CoV-2感染生物标志物的诊断性能。通过流式细胞术对两组独立的住院患者进行PLT-EVs定量检测，其中一组为Sars-CoV-2阳性（n = 69），另一组为Sars-CoV-2阴性（n = 62），还有健康对照组。通过受试者工作特征（ROC）曲线评估PLT-EVs的诊断性能。与Sars-CoV-2阴性患者或健康对照组相比，Sars-CoV-2阳性患者的PLT-EVs计数更高。对合并队列的ROC分析显示，曲线下面积（AUC）= 0.79，最佳截断值为1472个EVs/μL，灵敏度为75%，特异性为74%。这些数据表明，PLT-EVs可能是一个值得进一步研究以测试其预测能力有趣的生物标志物。
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