用于灵敏且选择性检测依折麦布的核壳磁性分子印迹聚合物基电化学传感器的研制
Development of core-shell magnetic molecularly imprinted polymer-based electrochemical sensor for sensitive and selective detection of ezetimibe.
作者信息
Hashkavayi Ayemeh Bagheri, Alizadeh Abdolhossein, Azimi Razieh, Peyrovi Moazameh, Raoof Jahan Bakhsh, Chun Honggu
机构信息
Department of Biomedical Engineering, Korea University, Seoul 02841, South Korea.
Department of Chemical Industry, Bushehr Branch, Technical and Vocational University, Bushehr, Iran.
出版信息
Heliyon. 2023 Jun 10;9(6):e17169. doi: 10.1016/j.heliyon.2023.e17169. eCollection 2023 Jun.
A sensitive electrochemical molecularly imprinted polymer (MIP) sensor was fabricated for detection of ezetimibe (Eze) as an effective cholesterol absorption inhibitor on the surface of a screen-printed carbon electrode based on a magnetic nanoparticle decorated with MIP (FeO@MIP). Placing the magnetic nanoparticle inside the MIP increases the biocompatibility, surface-to-volume ratio, and sensitivity of the sensor. Methacrylic acid (MAA) was used as a monomer, ethylene glycol dimethacrylate (EGDMA) as a cross-linker, and Eze as a template. The fabricated FeO@MIP was characterized using Fourier-transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM), and scanning electron microscopy (SEM). Detection of Eze was achieved by differential pulse voltammetry. Using this sensor, Eze can be sensitively detected in the range of 1.0 nM-10 μM and detection limit of 0.7 nM. In addition, we have shown that the proposed sensor successfully detects different concentrations of Eze in human serum samples and thus proves its practical application.
制备了一种灵敏的电化学分子印迹聚合物（MIP）传感器，用于检测依泽替米贝（Eze），它是一种有效的胆固醇吸收抑制剂。该传感器基于用MIP修饰的磁性纳米颗粒（FeO@MIP），制备于丝网印刷碳电极表面。将磁性纳米颗粒置于MIP内部可提高传感器的生物相容性、表面体积比和灵敏度。以甲基丙烯酸（MAA）为单体，乙二醇二甲基丙烯酸酯（EGDMA）为交联剂，Eze为模板。采用傅里叶变换红外光谱（FTIR）、透射电子显微镜（TEM）和扫描电子显微镜（SEM）对制备的FeO@MIP进行了表征。通过差分脉冲伏安法实现对Eze的检测。使用该传感器，可在1.0 nM - 10 μM范围内灵敏检测Eze，检测限为0.7 nM。此外，我们还表明所提出的传感器成功检测了人血清样品中不同浓度的Eze，从而证明了其实际应用价值。
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