Motia Soukaina, Bouchikhi Benachir, El Bari Nezha
Biotechnology Agroalimentary and Biomedical Analysis Group, Department of Biology, Faculty of Sciences, Moulay Ismaïl University of Meknes, B.P. 11201, Zitoune, Meknes, Morocco; Sensor Electronic & Instrumentation Group, Department of Physics, Faculty of Sciences, Moulay Ismaïl University of Meknes, B.P. 11201, Zitoune, Meknes, Morocco.
Sensor Electronic & Instrumentation Group, Department of Physics, Faculty of Sciences, Moulay Ismaïl University of Meknes, B.P. 11201, Zitoune, Meknes, Morocco.
Talanta. 2021 Feb 1;223(Pt 1):121689. doi: 10.1016/j.talanta.2020.121689. Epub 2020 Sep 24.
One of the most widely used synthetic antioxidants in food, butylated hydroxyanisole (BHA) has raised serious concerns due to its potential toxic effects on human health. Hence, elaboration of simple, effective and sensitive methods for BHA detection is pressing. In this regards, the present research work highlights a facile, simple, and fast synthesis approach for the development of an electrochemical sensor for the analysis of BHA in foodstuffs. In this study, the chitosan (CS) capped with gold nanoparticles (AuNPs) were self-assembled on a screen-printed carbon electrode (SPCE) and complete the elaboration of the molecularly imprinted polymer (MIP) sensor in the presence of BHA as templates. The electrochemical behaviour of the MIP sensor was investigated by using electrochemical impedance spectroscopy (EIS), differential pulse voltammetry (DPV), and cyclic voltammetry (CV). Similarly, the morphology of the electrodes surface of the different elaboration steps was characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, and atomic force microscopy (AFM). In addition, the obtained results demonstrate satisfactory sensitivity and selectivity to BHA compared to interfering species, including ascorbic acid and citric acid. Under optimal experimental conditions, the MIP sensor exhibits responses proportional to concentrations over a range of 0.01-20 μg mL, with a low detection limit (LOD) of 0.001 μg mL (signal-to-noise ratio S/N = 3). Besides, the reproducibility, stability, and repeatability of the MIP sensor were proven. Taking into account all these outcomes, the MIP sensor well demonstrates its ability towards the determination of BHA in food samples with a relative standard deviation (RSD ≤ 8%). Spectrophotometry was utilized as a validation method. Partial least squares (PLS) prediction models were constructed from the MIP sensor and spectrophotometer data with a regression coefficient (R = 0.99). According to the achieved outcomes, the MIP sensor could be a viable tool for food control.
丁基羟基茴香醚（BHA）是食品中使用最广泛的合成抗氧化剂之一，因其对人体健康的潜在毒性作用而引发了严重关注。因此，开发简单、有效且灵敏的BHA检测方法迫在眉睫。在这方面，本研究工作突出了一种简便、简单且快速的合成方法，用于开发一种用于分析食品中BHA的电化学传感器。在本研究中，用金纳米粒子（AuNPs）包覆的壳聚糖（CS）自组装在丝网印刷碳电极（SPCE）上，并在以BHA为模板的情况下完成分子印迹聚合物（MIP）传感器的制备。通过电化学阻抗谱（EIS）、差分脉冲伏安法（DPV）和循环伏安法（CV）研究了MIP传感器的电化学行为。同样，通过扫描电子显微镜（SEM）、傅里叶变换红外（FTIR）光谱和原子力显微镜（AFM）对不同制备步骤的电极表面形态进行了表征。此外，与包括抗坏血酸和柠檬酸在内的干扰物质相比，所得结果表明该MIP传感器对BHA具有令人满意的灵敏度和选择性。在最佳实验条件下，MIP传感器在0.01 - 20 μg mL范围内呈现与浓度成正比的响应，检测限低至0.001 μg mL（信噪比S/N = 3）。此外，还证明了MIP传感器的重现性、稳定性和重复性。考虑到所有这些结果，MIP传感器很好地展示了其测定食品样品中BHA的能力，相对标准偏差（RSD≤8%）。采用分光光度法作为验证方法。根据MIP传感器和分光光度计数据构建了偏最小二乘（PLS）预测模型，回归系数（R = 0.99）。根据所取得的结果，MIP传感器可能是食品检测的一种可行工具。
