Hesketh-Best Poppy J, January Grant G, Koch Matthew J, Warburton Philip J, Howell Kerry L, Upton Mathew
School of Biological and Marine Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA, United Kingdom.
School of Biomedical Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA, United Kingdom.
FEMS Microbes. 2023 Feb 22;4:xtad005. doi: 10.1093/femsmc/xtad005. eCollection 2023.
Global antimicrobial resistance is a health crisis that can change the face of modern medicine. Exploring diverse natural habitats for bacterially-derived novel antimicrobial compounds has historically been a successful strategy. The deep-sea presents an exciting opportunity for the cultivation of taxonomically novel organisms and exploring potentially chemically novel spaces. In this study, the draft genomes of 12 bacteria previously isolated from the deep-sea sponges and sp. are investigated for the diversity of specialized secondary metabolites. In addition, early data support the production of antibacterial inhibitory substances produced from a number of these strains, including activity against clinically relevant pathogens , and . Draft whole-genomes are presented of 12 deep-sea isolates, which include four potentially novel strains: sp. PP-21, sp. DK15, sp. PP-33, and sp. M4NT. Across the 12 draft genomes, 138 biosynthetic gene clusters were detected, of which over half displayed less than 50% similarity to known BGCs, suggesting that these genomes present an exciting opportunity to elucidate novel secondary metabolites. Exploring bacterial isolates belonging to the phylum Actinomycetota, Pseudomonadota, and Bacillota from understudied deep-sea sponges provided opportunities to search for new chemical diversity of interest to those working in antibiotic discovery.
全球抗菌药物耐药性是一场可能改变现代医学面貌的健康危机。从历史上看，探索各种自然栖息地以寻找细菌衍生的新型抗菌化合物一直是一种成功的策略。深海为培养分类学上新颖的生物和探索潜在的化学新领域提供了令人兴奋的机会。在本研究中，对先前从深海海绵和 种中分离出的12种细菌的基因组草图进行了研究，以了解其特殊次生代谢产物的多样性。此外，早期数据支持其中一些菌株产生抗菌抑制物质，包括对临床相关病原体 和 的活性。本文展示了12株深海分离株的全基因组草图，其中包括4株潜在的新菌株： 种PP-21、 种DK15、 种PP-33和 种M4NT。在这12个基因组草图中，共检测到138个生物合成基因簇，其中超过一半与已知的生物合成基因簇相似度低于50%，这表明这些基因组为阐明新型次生代谢产物提供了令人兴奋的机会。从研究较少的深海海绵中探索属于放线菌门、假单胞菌门和芽孢杆菌门的细菌分离株，为那些从事抗生素发现工作的人寻找新的化学多样性提供了机会。
