School of Biomedical Sciences, University of Plymouth, Plymouth PL4 8AA, UK.
School of Biological and Marine Sciences, University of Plymouth, Plymouth PL4 8AA, UK.
Microbiology (Reading). 2021 Dec;167(12). doi: 10.1099/mic.0.001123.
Access to deep-sea sponges brings with it the potential to discover novel antimicrobial candidates, as well as novel cold- and pressure-adapted bacteria with further potential clinical or industrial applications. In this study, we implemented a combination of different growth media, increased pressure and high-throughput techniques to optimize recovery of isolates from two deep-sea hexactinellid sponges, and sp., in the first culture-based microbial analysis of these two sponges. Using 16S rRNA gene sequencing for isolate identification, we found a similar number of cultivable taxa from each sponge species as well as improved recovery of morphotypes from at 22-25 °C compared to other temperatures, which allows a greater potential for screening for novel antimicrobial compounds. Bacteria recovered under conditions of increased pressure were from the phyla , and , except at 4 %O/5 bar, when the phylum was not observed. Cultured isolates from both sponge species displayed antimicrobial activity against and .
深海海绵的获取带来了发现新型抗菌候选物的潜力，以及具有进一步潜在临床或工业应用的新型耐冷和耐压细菌。在这项研究中，我们采用了不同的生长培养基组合、增加压力和高通量技术，以优化从两种深海六射珊瑚海绵（ 和 sp.）中分离培养物的回收，这是对这两种海绵进行的首次基于培养的微生物分析。通过 16S rRNA 基因测序进行分离物鉴定，我们发现从每个海绵物种中获得的可培养分类群数量相似，并且与其他温度相比，在 22-25°C 下从 中获得的形态类型的回收得到了改善，这为筛选新型抗菌化合物提供了更大的潜力。在增加压力条件下回收的细菌来自门 、 和 ，但在 4%O/5 巴时除外，此时未观察到门 。从两种海绵物种培养的分离物均对 和 显示出抗菌活性。
