Sorbonne Université, PSL Research University, CNRS, Laboratoire Physique et Etudes des Matériaux, ESPCI Paris, Paris, France.
Imagine Optic, Orsay, France.
J Biomed Opt. 2023 Jun;28(6):066501. doi: 10.1117/1.JBO.28.6.066501. Epub 2023 Jun 16.
Adaptive optics (AO) has been implemented on several microscopy setups and has proven its ability to increase both signal and resolution. However, reported configurations are not suited for fast imaging of live samples or are based on an invasive or complex implementation method.
Provide a fast aberration correction method with an easy to implement AO module compatible with light-sheet fluorescence microscopy (LSFM) for enhanced imaging of live samples.
Development of an AO add-on module for LSFM based on direct wavefront sensing without requiring a guide star using an extended-scene Shack-Hartmann wavefront sensor. The enhanced setup uses a two-color sample labeling strategy to optimize the photon budget.
Fast AO correction of in-depth aberrations in an adult brain enables doubling the contrast when imaging with either cell reporters or calcium sensors for functional imaging. We quantify the gain in terms of image quality on different functional domains of sleep neurons in the brain at various depths and discuss the optimization of key parameters driving AO.
We developed a compact AO module that can be integrated into most of the reported light-sheet microscopy setups, provides significant improvement of image quality and is compatible with fast imaging requirements such as calcium imaging.
自适应光学（AO）已经在多个显微镜设置中得到实施，并已证明其具有提高信号和分辨率的能力。然而，报告的配置不适合快速成像活样本，或者基于侵入性或复杂的实现方法。
提供一种快速像差校正方法，该方法具有易于实施的 AO 模块，与光片荧光显微镜（LSFM）兼容，用于增强活样本的成像。
基于直接波前感测，为 LSFM 开发 AO 附加模块，无需使用扩展场景 Shack-Hartmann 波前传感器引导星。增强后的设置使用双色样品标记策略来优化光子预算。
对成年大脑中的深度像差进行快速 AO 校正，可使细胞报告器或钙传感器进行功能成像时的对比度提高一倍。我们根据不同深度的睡眠神经元的不同功能域的图像质量对增益进行量化，并讨论了驱动 AO 的关键参数的优化。
我们开发了一种紧凑的 AO 模块，可集成到大多数报告的光片显微镜设置中，显著提高图像质量，并与钙成像等快速成像要求兼容。
