Department of Chemistry, Faculty of Science, Atatürk University, 25240 Erzurum, Türkiye.
Program of Occupational Health and Safety, Vocational College of Technical Sciences, Atatürk University, 25240 Erzurum, Türkiye.
Org Biomol Chem. 2023 Jul 5;21(26):5406-5412. doi: 10.1039/d3ob00602f.
In this study, a new tetraphenylethene derivative bearing triazole and pyridine moieties (TPE-TAP) was synthesized using click chemistry. The fluorescence sensing properties of TPE-TAP were investigated in almost 100% aqueous media. For this purpose, first of all, structural characterization of the newly synthesized compound TPE-TAP was performed by NMR and HRMS analyses. Then, the optical behavior of TPE-TAP was investigated in different ratios of a THF-water mixture (0-98%). The obtained results showed that the best fluorescence for TPE-TAP was observed in the presence of 98% water in the medium. Then, the ion selectivity of TPE-TAP was determined using 19 different cations in the THF-water medium (2 : 98 v/v%). It was observed that among the cations studied, only Fe quenched the fluorescence of TPE-TAP. The detection limit and binding constant values of TPE-TAP for Fe were calculated as 1.3 μM and 26.65 M, respectively, from the graph results of the decrease in the fluorescence intensity of TPE-TAP in the presence of Fe at different concentrations. In addition, the study investigating the selectivity of TPE-TAP in the presence of 18 cations other than Fe showed that none of the studied cations interfered with Fe. Practical application of TPE-TAP was also carried out using a commercial iron drug. All results showed that TPE-TAP is a fluorometric sensor highly selective, sensitive, and suitable for practical application for Fe ions in aqueous medium.
在这项研究中，使用点击化学合成了一种带有三唑和吡啶部分的新四苯乙烯衍生物（TPE-TAP）。在近 100%的水介质中研究了 TPE-TAP 的荧光传感特性。为此，首先通过 NMR 和 HRMS 分析对新合成的化合物 TPE-TAP 的结构进行了表征。然后，在不同比例的 THF-水混合物（0-98%）中研究了 TPE-TAP 的光学行为。结果表明，在介质中存在 98%的水时，TPE-TAP 的荧光最佳。然后，在 THF-水介质（2:98v/v%）中使用 19 种不同的阳离子测定了 TPE-TAP 的离子选择性。观察到，在所研究的阳离子中，只有 Fe 猝灭了 TPE-TAP 的荧光。根据 TPE-TAP 在不同浓度的 Fe 存在下荧光强度降低的图谱结果，计算出 TPE-TAP 对 Fe 的检测限和结合常数值分别为 1.3 μM 和 26.65 M。此外，研究了 TPE-TAP 在除 Fe 以外的 18 种阳离子存在下的选择性，结果表明，研究的阳离子都没有干扰 Fe。还使用商业铁药物进行了 TPE-TAP 的实际应用研究。所有结果表明，TPE-TAP 是一种在水介质中对 Fe 离子具有高选择性、高灵敏度和实用应用潜力的荧光传感器。
