National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, 130000, China.
National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China.
Dig Dis Sci. 2023 Aug;68(8):3283-3292. doi: 10.1007/s10620-023-07990-6. Epub 2023 Jun 19.
BACKGROUND/AIMS: To explore the protective effects and therapeutic mechanism of Esomeprazole (PPI), polaprezinc granule (PZ), and PPI + PZ on reflux esophagitis (RE) in the rat model.
Wistar rats were randomly divided into 9 groups, which contain the control group, the acid cessation group (0.7% HCl, Q3D × 4), and the acid persistence group (0.7% HCl, Q3D × 11). PPI was administered by gavage at 8 mg·kg body weight and PZ was administered by gavage at 120 mg·kg body weight once a day for 15 days. The gastric cardia tissue of the feeding tube was observed under the light microscope, and the levels of interleukin-8 (IL-8) and prostaglandin E2 (PGE2) were measured by ELISA. The expression of EGFR, Akt, p-Akt, and p-mTOR was detected by Western blot.
The ELISA results showed that the levels of IL-8 and PGE2 were significantly increased in the model group, but decreased in all groups after treatment. In the acid cessation group, PZ treatment had the most significant effect on reducing IL-8 levels and PPI + PZ treatment had the most significant effect on reducing PGE2 levels. In the acid persistence group, the PPI treatment had the most significant effect on reducing the levels of IL-8 and PGE2, and the PZ treatment could also significantly reduce their levels, close to the normal value. Western blot results showed that the expression of PI3K/Akt/mTOR pathway protein was increased in the model group, while its expression was decreased after treatment.
Polaprezinc has a significant therapeutic effect on RE in rats, which can reduce the levels of IL-8 and PGE2 and downregulate the expression of PI3K/Akt/mTOR signal pathway protein. The efficacy of polaprezinc in the treatment of reflux esophagitis is comparable to that of PPI, and the combination of them is more effective in the reflux esophagitis treatment.
背景/目的：探讨埃索美拉唑（PPI）、聚普瑞锌颗粒（PZ）和 PPI+PZ 对反流性食管炎（RE）大鼠模型的保护作用和治疗机制。
将 Wistar 大鼠随机分为 9 组，分别为对照组、酸抑制组（0.7%HCl，Q3D×4）和酸持续组（0.7%HCl，Q3D×11）。通过灌胃给予 PPI 8mg·kg 体重，每天 1 次，给予 PZ 120mg·kg 体重，连续 15 天。观察饲管胃贲门组织在光镜下的变化，并通过 ELISA 测定白细胞介素-8（IL-8）和前列腺素 E2（PGE2）的水平。通过 Western blot 检测表皮生长因子受体（EGFR）、Akt、p-Akt 和 p-mTOR 的表达。
ELISA 结果显示，模型组 IL-8 和 PGE2 水平显著升高，治疗后均降低。在酸抑制组中，PZ 治疗降低 IL-8 水平的效果最显著，PPI+PZ 治疗降低 PGE2 水平的效果最显著。在酸持续组中，PPI 治疗降低 IL-8 和 PGE2 水平的效果最显著，PZ 治疗也能显著降低其水平，接近正常值。Western blot 结果显示，模型组 PI3K/Akt/mTOR 通路蛋白表达增加，治疗后表达降低。
聚普瑞锌对大鼠 RE 有显著的治疗作用，可降低 IL-8 和 PGE2 水平，下调 PI3K/Akt/mTOR 信号通路蛋白的表达。聚普瑞锌治疗反流性食管炎的疗效与 PPI 相当，两者联合治疗更有效。
