Lv Mi, Huang Kai-Yue, Wang Xiao-Kang, Wang Yu-Xi, Qiao Xi-Yun, Che Hui, Lv Lin, Wang Feng-Yun
Xiyuan Hospital, China Academy of Traditional Chinese Medicine, No. 1 Xiyuan Playground, Haidian District, Beijing, 100091, China.
Beijing University of Chinese Medicine, Beisanhuan East Road, Chaoyang District, Beijing, 100029, China.
Dig Dis Sci. 2025 Apr 1. doi: 10.1007/s10620-025-09022-x.
The differences in the animal model construction of different subtypes of gastroesophageal reflux disease (GERD) have not been clearly demonstrated at present. We aim to reveal the characteristics and differences between them.
The literature related to GERD animal model construction in the past decade was searched and data on animal strains, modeling modes, modeling cycles, and detection indices were extracted, and the results were presented by using descriptive statistical methods of frequency and relative frequency.
88 papers finally met the criteria. Sprague-Dawley (68.25%) rats were most often used to induce reflux esophagitis (RE), whereas Swiss mice (50.00%) and C57BL/6 mice (57.89%) for non-erosive reflux disease (NERD) and Barrett's esophagus (BE), respectively. RE and NERD were most frequently constructed using fore-stomach-glandular transition ligation together with pyloric insufficiency (37.68%, 50.00%), yet their median modeling cycles were 14 and 7 days, respectively. BE was most frequently constructed using L2-IL-1β transgenic mice (27.27%), and the median modeling cycle over 270 days. Determining esophageal mucosal permeability was common in NERD, while finding intestinal chemotaxis markers, squamous epithelium, and columnar epithelium was common in BE. In animal models of RE, researchers tended to look for markers associated with the inflammatory response and oxidative stress.
The induction methods vary among the animal models of GERD's three subtypes. Inflammatory stimulation is crucial for inducing RE and BE, differing in modeling cycle. In contrast, Visceral hypersensitivity draws more attention in NERD animal models, reflecting researchers' thoughts on distinct pathogenesis.
目前不同亚型胃食管反流病（GERD）动物模型构建的差异尚未明确阐明。我们旨在揭示它们之间的特征和差异。
检索过去十年中与GERD动物模型构建相关的文献，提取动物品系、建模方式、建模周期和检测指标的数据，并采用频率和相对频率的描述性统计方法呈现结果。
最终88篇论文符合标准。Sprague-Dawley大鼠（68.25%）最常用于诱导反流性食管炎（RE），而瑞士小鼠（50.00%）和C57BL/6小鼠（57.89%）分别用于非糜烂性反流病（NERD）和巴雷特食管（BE）的模型构建。RE和NERD最常采用前胃-腺过渡结扎联合幽门功能不全构建（分别为37.68%、50.00%），但其建模周期中位数分别为14天和7天。BE最常采用L2-IL-1β转基因小鼠构建（27.27%），建模周期中位数超过270天。在NERD中，测定食管黏膜通透性较为常见，而在BE中，寻找肠道趋化标记物、鳞状上皮和柱状上皮较为常见。在RE动物模型中，研究人员倾向于寻找与炎症反应和氧化应激相关的标记物。
GERD三种亚型的动物模型诱导方法各不相同。炎症刺激对诱导RE和BE至关重要，建模周期有所不同。相比之下，内脏高敏感性在NERD动物模型中更受关注，反映了研究人员对不同发病机制的思考。
