University of Franca (UNIFRAN), Avenida Dr Armando Sales de Oliveira, 201, Parque Universitário, Franca, São Paulo 14404-600, Brazil.
Department of Entomology, Rutgers University, P.E. Marucci Center, 125A Lake Oswego Road, Chatsworth, NJ 08019, USA.
J Insect Sci. 2023 May 1;23(3). doi: 10.1093/jisesa/iead038.
Green lacewings (Neuroptera: Chrysopidae) are predators commonly found in coffee plantations in Brazil that can serve as important biological control agents against insect pests such as the coffee leaf miner, Leucoptera coffeella (Lepidoptera: Lyonetiidae). However, the efficacy of different lacewing species in controlling L. coffeella needs to be evaluated before they are used in augmentative biological control programs. Here, laboratory experiments were conducted to investigate the effects of the L. coffeella developmental stage on the functional response of 3 species of green lacewings: Chrysoperla externa, Ceraeochrysa cincta, and Ceraeochrysa cornuta. The attack rate, handling time, and the number of prey attacked during 24 h with different densities (1, 2, 4, 8, 16, 32, and 64 individuals) of either L. coffeella larvae or pupae were recorded for each of the 3 lacewing species. Based on logistic regression models, all 3 predators showed a Type II functional response when consuming both larvae and pupae of L. coffeella. All 3 species also had similar attack rates (0.0091 larva/h and 0.0095 pupa/h), handling times (3.5 and 3.7 h for larvae and pupae, respectively), and estimated number of prey attacked during the observation period (6.9 larvae and 6.6 pupae) for L. coffeella larvae and pupae. Therefore, our laboratory studies show that the 3 green lacewings Ch. externa, Ce. cincta, and Ce. cornuta have potential for the biological control of L. coffeella, although these results need to be confirmed under field conditions. These findings have implications for the selection of lacewings for augmentative L. coffeella biocontrol.
绿叶蝉（Neuroptera：Chrysopidae）是巴西咖啡种植园中常见的捕食者，可用作防治咖啡潜叶蛾（Lepidoptera：Lyonetiidae）等害虫的重要生物防治剂。然而，在将不同的草蛉物种用于补充生物防治计划之前，需要评估它们对 L. coffeella 的控制效果。在这里，进行了实验室实验，以研究 L. coffeella 发育阶段对 3 种绿叶蝉（Chrysoperla externa、Ceraeochrysa cincta 和 Ceraeochrysa cornuta）功能反应的影响。记录了每只捕食者在不同密度（1、2、4、8、16、32 和 64 只）的 L. coffeella 幼虫或蛹期 24 小时内的攻击率、处理时间和攻击的猎物数量。对于 3 种草蛉，均采用逻辑回归模型来记录。当消耗 L. coffeella 的幼虫和蛹时，所有 3 种捕食者均表现出 II 型功能反应。3 种捕食者对 L. coffeella 幼虫和蛹的攻击率（分别为 0.0091 只/小时和 0.0095 只/小时）、处理时间（幼虫和蛹分别为 3.5 和 3.7 小时）和估计在观察期间攻击的猎物数量（6.9 只幼虫和 6.6 只蛹）也相似。因此，我们的实验室研究表明，3 种绿叶蝉 Ch. externa、Ce. cincta 和 Ce. cornuta 具有防治 L. coffeella 的潜力，尽管这些结果需要在田间条件下得到证实。这些发现对选择用于补充 L. coffeella 生物防治的草蛉具有重要意义。
