Université Paris-Cité, Innovative Therapies in Hemostasis, Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France; Hematology Department, Assistance Publique-Hôpitaux de Paris (AP-HP), Georges Pompidou European Hospital, Paris, France.
National Heart and Lung Institute, Imperial College London, London, UK.
J Thromb Haemost. 2023 Sep;21(9):2611-2619. doi: 10.1016/j.jtha.2023.06.014. Epub 2023 Jun 17.
Assessment of endothelial colony-forming cell (ECFC) number and vasculogenic properties is crucial for exploring vascular diseases and regeneration strategies. A previous survey of the Scientific and Standardization Committee on Vascular Biology of the International Society on Thrombosis and Haemostasis clarified key methodological points but highlighted a lack of standardization associated with ECFC culture.
The aim of this study was to provide expert consensus guidance on ECFC isolation and culture.
We surveyed 21 experts from 10 different countries using a questionnaire proposed during the 2019 International Society on Thrombosis and Haemostasis Congress in Melbourne (Australia) to attain a consensus on ECFC isolation and culture.
We report here the consolidated results of the questionnaire. There was agreement on several general statements, mainly the technical aspects of ECFC isolation and cell culture. In contrast, on the points concerning the definition of a colony of ECFCs, the quantification of ECFCs, and the estimation of their age (in days or number of passages), the expert opinions were widely dispersed.
Our survey clearly indicates an unmet need for rigorous standardization, multicenter comparison of results, and validation of ECFC isolation and culture procedures for clinical laboratory practice and robustness of results. To this end, we propose a standardized protocol for the isolation and expansion of ECFCs from umbilical cord and adult peripheral blood.
评估内皮祖细胞（endothelial colony-forming cell，ECFC）数量和血管生成特性对于探索血管疾病和再生策略至关重要。国际血栓与止血学会血管生物学科学与标准化委员会的一项先前调查明确了关键的方法要点，但突出了与 ECFC 培养相关的缺乏标准化问题。
本研究旨在为 ECFC 分离和培养提供专家共识指导。
我们在 2019 年于澳大利亚墨尔本举行的国际血栓与止血学会大会期间，使用问卷调查了来自 10 个不同国家的 21 位专家，以就 ECFC 分离和培养达成共识。
本研究报告了问卷调查的综合结果。专家们就 ECFC 分离和细胞培养的技术方面达成了一些共识，但在 ECFC 集落的定义、ECFC 的定量以及其年龄（以天数或传代数计）等方面的观点存在广泛分歧。
我们的调查清楚地表明，需要严格标准化、多中心比较结果，并验证 ECFC 分离和培养程序在临床实验室实践中的稳健性和结果的可靠性。为此，我们提出了一种从脐带和成人外周血中分离和扩增 ECFC 的标准化方案。
