College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi Province 712100, China.
College of Resources Environment Science and Technology, Hubei University of Science and Technology, Xianning 437100, Hubei, China.
Bioresour Technol. 2023 Sep;384:129358. doi: 10.1016/j.biortech.2023.129358. Epub 2023 Jun 17.
Bacterial communities were dynamically tracked at four stages of biochar-driven sheep manure pile composting, and the co-occurrence networks with keystone taxa were established. The succession of bacterial community obvious varied during the composting process, Proteobacteria predominant in initial stage (39%) then shifted into Firmicutes in thermophilic (41%) and mesophilic (27%) stages, finally the maturation stage dominant by Bacteroidota (26%). Visualizations of bacterial co-occurrence networks demonstrate more cooperative mutualism and complex interactions in the thermophilic and mesophilic phases. Noticeably, the 7.5 and 10% biochar amended composts shown highest connections (736 and 663 total links) and positive cooperation (97.37 and 97.13% positive link) as well as higher closeness centrality and betweenness centrality of keystone taxa. Overall, appropriate biochar addition alters bacterial community succession and strengthens connection between keystone taxa and other bacteria, with 7.5 and 10% biochar amended composts has intense mutualistic symbiosis among bacterial communities.
在生物炭驱动羊粪堆肥的四个阶段动态跟踪细菌群落，并建立具有关键分类群的共生网络。在堆肥过程中，细菌群落的演替明显不同，在初始阶段（39%）以变形菌门为主，然后在高温（41%）和中温（27%）阶段转变为厚壁菌门，最后成熟阶段以拟杆菌门（26%）为主。细菌共生网络的可视化显示，在高温和中温阶段，共生关系更加密切，相互作用更加复杂。值得注意的是，添加 7.5%和 10%生物炭的堆肥显示出最高的连接数（736 和 663 条总链接）和阳性合作（97.37%和 97.13%阳性链接），以及更高的关键分类群的接近中心度和中介中心度。总的来说，适当添加生物炭会改变细菌群落的演替，并增强关键分类群与其他细菌之间的联系，添加 7.5%和 10%生物炭的堆肥中细菌群落之间存在强烈的互利共生关系。
