胱氨酸和茶氨酸混合物对全身 X 射线照射后大鼠慢性存活率和肿瘤发生率的影响。
The effects of cystine and theanine mixture on the chronic survival rate and tumor incidence of rats after total body X-ray irradiation†.
机构信息
Division of Strategic Collaborative Research, Center for Promotion of Collaborative Research on Radiation and Environment Health Effects, Atomic Bomb Disease Institute, Nagasaki University, 1-12-4 Sakamoto, Nagasaki, Nagasaki 852-8523, Japan.
Department of Tumor and Diagnostic Pathology, Atomic Bomb Disease Institute, Nagasaki University, 1-12-4 Sakamoto, Nagasaki, Nagasaki 852-8523, Japan.
出版信息
J Radiat Res. 2023 Jul 18;64(4):738-742. doi: 10.1093/jrr/rrad047.
Cystine and theanine (CT), an amino acid mixture, provides the substrates cysteine and glutamic acid that promote glutathione synthesis. We previously reported that CT pre-treatment significantly improved the acute survival rate and reduced acute radiation injury of the small intestine and bone marrow of rats after 5 Gy of total body X-ray irradiation. To examine the long-term effects of CT administration after irradiation, we investigated the effects of CT pre-treatment and pre- and post-treatment on the chronic survival rate and solid tumor (spleen, skin and subcutis, and thyroid) incidence after irradiation using 7-week-old male Wistar rats. CT pre-treatment of 280 mg/kg was administered orally for 5 days before 5 Gy irradiation, and CT pre- and post-treatment was administered 5 days before and 5 days after irradiation. A 0.5% carboxymethyl cellulose solution was administered as a control. The chronic survival rate of the pre-treated rats was higher than that of the control rats at 441 days after irradiation (40 vs 8.1%, P = 0.011). However, the survival rate did not significantly differ between the pre- and post-treatment and control rats at 467 days after irradiation (33.8 vs 30.2%, P = 0.792). In addition, more solid tumors, especially subcutis sarcomas, were observed in the pre-treatment rats (26.1%, 6/23) than in the control rats (4.5%, 1/22) after irradiation. Therefore, pre-administration of CT improves the chronic survival rate after irradiation; however, the occurrence of solid tumors was not suppressed.
半胱氨酸和茶氨酸（CT）是一种氨基酸混合物，可提供促进谷胱甘肽合成的半胱氨酸和谷氨酸底物。我们之前报道过，CT 预处理可显著提高 5Gy 全身 X 射线照射后大鼠小肠和骨髓的急性存活率并减轻急性放射损伤。为了研究照射后 CT 给药的长期影响，我们使用 7 周龄雄性 Wistar 大鼠研究了 CT 预处理和照射前及照射后处理对照射后慢性存活率和实体瘤（脾脏、皮肤和皮下组织、甲状腺）发生率的影响。在 5Gy 照射前 5 天，经口给予 280mg/kg 的 CT 预处理，照射前和照射后各给予 5 天 CT。以 0.5%羧甲基纤维素溶液作为对照。照射后 441 天时，预处理大鼠的慢性存活率高于对照组（40% vs 8.1%，P=0.011）。然而，照射后 467 天时，预处理组和对照组之间的存活率没有显著差异（33.8% vs 30.2%，P=0.792）。此外，照射后预处理大鼠比对照组大鼠观察到更多的实体瘤，特别是皮下肉瘤（26.1%，6/23）比对照组（4.5%，1/22）多。因此，CT 预处理可提高照射后的慢性存活率；然而，并未抑制实体瘤的发生。
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