Farrand S K, Dessaux Y
J Bacteriol. 1986 Aug;167(2):732-4. doi: 10.1128/jb.167.2.732-734.1986.
Octopine or nopaline Ti plasmids, or clones encoding their occ or noc loci, allowed proline auxotrophs of Agrobacterium tumefaciens to utilize the appropriate arginyl opine as a proline substitute. Arginine and ornithine substituted for proline only if the occ or noc loci were induced or made constitutive by mutation. These results support a report demonstrating a Ti plasmid-encoded activity in A. tumefaciens which cyclizes ornithine to proline.
章鱼碱或胭脂碱Ti质粒，或编码其occ或noc位点的克隆，使根癌土壤杆菌的脯氨酸营养缺陷型能够利用相应的精氨酰章鱼碱作为脯氨酸替代物。只有当occ或noc位点通过突变被诱导或组成型表达时，精氨酸和鸟氨酸才能替代脯氨酸。这些结果支持了一份报告，该报告证明了根癌土壤杆菌中一种由Ti质粒编码的将鸟氨酸环化生成脯氨酸的活性。
