Qureshi A A, Burger W C, Peterson D M, Elson C E
J Biol Chem. 1986 Aug 15;261(23):10544-50.
Purification of the oily, nonpolar fraction of high protein barley (Hordeum vulgare L.) flour by high pressure liquid chromatography yielded 10 major components, two (I, II) of which were potent inhibitors of cholesterogenesis in vivo and in vitro. The addition of purified inhibitor I (2.5-20 ppm) to chick diets significantly decreased hepatic cholesterogenesis and serum total and low density lipoprotein cholesterol and concomitantly increased lipogenic activity. The high resolution mass spectrometric analysis and measurement of different peaks of inhibitor I gave a molecular ion at m/e 424 (C29H44O2) and main peaks at m/e 205, 203, and 165 corresponding to C13H17O2, C13H15O2, and C10H13O2 moieties, respectively. which are characteristic of d-alpha-tocotrienol. This identification was confirmed against synthetic samples. The tocotrienols are widely distributed in the plant kingdom and differ from tocopherols (vitamin E) only in three double bonds in the isoprenoid chain which appear to be essential for the inhibition of cholesterogenesis.
通过高压液相色谱法对高蛋白大麦（Hordeum vulgare L.）面粉的油性非极性部分进行纯化，得到了10种主要成分，其中两种（I、II）在体内和体外均为胆固醇生成的有效抑制剂。在雏鸡日粮中添加纯化的抑制剂I（2.5 - 20 ppm）可显著降低肝脏胆固醇生成以及血清总胆固醇和低密度脂蛋白胆固醇，并同时提高脂肪生成活性。对抑制剂I的不同峰进行高分辨率质谱分析和测量，得到分子离子峰m/e 424（C29H44O2），以及分别对应于C13H17O2、C13H15O2和C10H13O2部分的主要峰m/e 205、203和165，这些是d-α-生育三烯酚的特征峰。该鉴定结果与合成样品进行了比对确认。生育三烯酚广泛分布于植物界，与生育酚（维生素E）的区别仅在于类异戊二烯链中有三个双键，这些双键似乎对抑制胆固醇生成至关重要。
