Kumar Sanjeev, Mondal Kalyan K, Ghoshal Thungri, Kulshreshtha Aditya, Sreenayana B, Lakshmi M Amrutha, Mrutyunjaya S, Rashmi E R, Kalaivanan N S, Kundu Aditi, Mani Chandra
Division of Plant Pathology, ICAR, Indian Agricultural Research Institute, New Delhi, India.
Division of Agricultural Chemicals, ICAR, Indian Agricultural Research Institute, New Delhi, India.
3 Biotech. 2023 Jul;13(7):239. doi: 10.1007/s13205-023-03660-6. Epub 2023 Jun 17.
Bacterial panicle blight (BPB) caused by (BG) has become significantly more prevalent in the rice-growing regions of North India. Based on virulence screening and in vitro quantification of toxoflavin, the BG strains were classified as hyper- (BG1 and BG3), moderate- (BG2, BG4, BG6, BG8, and BG9), and hypo- (BG5, BG7, and BG10) virulent. Plant inoculation assays with cell-free culture filtrate revealed strains with higher toxoflavin-producing ability had higher virulence. Based on 16S rRNA sequence, 6 isolates from Uttar Pradesh were grouped in clad C1; whereas, clad C2 exhibited 4 isolates, two each from Delhi and Uttar Pradesh. Strain BG1 being the most virulent Indian strain from Uttar Pradesh was further profiled for 11 genes. We found all the 11 genes present in strain BG1. In cluster, all genes showed high similarity to BGR1 except , whereas in cluster , and shared maximum similarity to 336gr-1. genes of BG1 exhibited homology as well as divergence with . The domain prediction and protein association network analysis indicated the possible involvement of genes in the toxoflavin biosynthesis. As per our knowledge, this is the first report in India on characterization of genes cluster in . Altogether, our study unravels a reliable method for identifying and characterizing using genes and its relationship with disease production.
The online version contains supplementary material available at 10.1007/s13205-023-03660-6.
由稻黄单胞菌（BG）引起的细菌性穗枯病（BPB）在印度北部的水稻种植区已变得明显更为普遍。基于对毒黄素的毒力筛选和体外定量分析，BG菌株被分类为高毒力（BG1和BG3）、中毒力（BG2、BG4、BG6、BG8和BG9）和低毒力（BG5、BG7和BG10）。用无细胞培养滤液进行的植物接种试验表明，毒黄素产生能力较高的菌株具有更高的毒力。基于16S rRNA序列，来自北方邦的6个分离株被归入进化枝C1；而进化枝C2有4个分离株，分别来自德里和北方邦各两个。菌株BG1是来自北方邦毒性最强的印度菌株，进一步对其11个基因进行了分析。我们发现菌株BG1中存在所有11个基因。在簇中，除了外，所有基因与BGR1显示出高度相似性，而在簇中，和与336gr - 1共享最大相似性。BG1的基因与表现出同源性以及差异。结构域预测和蛋白质关联网络分析表明基因可能参与毒黄素的生物合成。据我们所知，这是印度关于稻黄单胞菌基因簇特征的首次报道。总之，我们的研究揭示了一种利用基因鉴定和表征稻黄单胞菌及其与病害发生关系的可靠方法。
在线版本包含可在10.1007/s13205 - 023 - 03660 - 6获取的补充材料。
