Division of Entomology, ICAR- Indian Agricultural Research Institute, New Delhi, 110012, India.
National Institute for Plant Biotechnology, ICAR- Indian Agricultural Research Institute, New Delhi, 110012, India.
Mol Biol Rep. 2023 Aug;50(8):6569-6578. doi: 10.1007/s11033-023-08605-z. Epub 2023 Jun 20.
Tribolium castaneum causes substantial damage to stored grains, leading to economic losses. The present study evaluates phosphine resistance in adult and larval stages of T. castaneum from north and northeast India, where continuous and long-term phosphine use in large-scale storage conditions intensifies resistance, posing risks to grain quality, safety, and industry profitability.
This study utilized T. castaneum bioassays and CAPS markers restriction digestion methodology to assess resistance. The phenotypic results indicated a lower LC value in larvae compared to adults, while the resistance ratio remained consistent across both stages. Similarly, the genotypic analysis revealed comparable resistance levels regardless of the developmental stage. We categorized the freshly collected populations based on resistance ratios, with Shillong showing weak resistance, Delhi and Sonipat displaying moderate resistance, and Karnal, Hapur, Moga, and Patiala exhibiting strong resistance to phosphine. Further validation by accessing findings and exploring the relationship between phenotypic and genotypic variations using Principal Component Analysis (PCA). This comprehensive study enhances our understanding of T. castaneum resistance levels, providing valuable insights for the development of targeted pest management strategies.
This study provides insights into the current phenotypic and genotypic resistance levels of T. castaneum in North and North East India. Understanding this is crucial for developing effective pest management strategies and future research on biological and physiological aspects of phosphine resistance in insects, enabling the formulation of effective management practices. Addressing phosphine resistance is vital for sustainable pest management and the long-term viability of the agricultural and food industries.
赤拟谷盗对储存的谷物造成严重损害，导致经济损失。本研究评估了来自印度北部和东北部的成虫和幼虫期赤拟谷盗对磷化氢的抗性，在大规模储存条件下，磷化氢的持续和长期使用加剧了抗性，对谷物质量、安全和产业盈利能力构成风险。
本研究利用赤拟谷盗生物测定和 CAPS 标记限制酶切方法评估抗性。表型结果表明幼虫的 LC 值低于成虫，而抗性比在两个阶段保持一致。同样，基因型分析显示无论发育阶段如何，抗性水平相当。我们根据抗性比对新收集的种群进行分类，其中 Shillong 表现出弱抗性，Delhi 和 Sonipat 显示出中度抗性，而 Karnal、Hapur、Moga 和 Patiala 对磷化氢表现出强抗性。通过访问发现并使用主成分分析（PCA）探索表型和基因型变异之间的关系进一步验证。这项综合研究增强了我们对赤拟谷盗抗性水平的理解，为制定有针对性的害虫管理策略提供了有价值的信息。
本研究提供了印度北部和东北部地区赤拟谷盗目前表型和基因型抗性水平的见解。了解这一点对于制定有效的害虫管理策略和未来对昆虫磷化氢抗性的生物学和生理学方面的研究至关重要，使有效的管理实践得以制定。解决磷化氢抗性问题对于可持续的害虫管理和农业及食品产业的长期生存能力至关重要。
