Department of Biomedicine and Prevention, University of Rome Tor Vergata, Via Montpellier 1, Rome, 00133, Italy.
Saint Camillus International University Of Health Sciences, Via di Sant'Alessandro 8, Rome, 00131, Italy.
Stem Cell Rev Rep. 2023 Oct;19(7):2274-2283. doi: 10.1007/s12015-023-10561-x. Epub 2023 Jun 20.
In the last two decades, considerable progress has been made in the derivation of mammalian germ cells from pluripotent stem cells such as Embryonic Stem Cells (ESCs) and induced Pluripotent Stem Cells (iPSCs). The pluripotent stem cells are generally first induced into pre-gastrulating endoderm/mesoderm-like status and then specified into putative primordial germ cells (PGCs) termed PGC-like cells (PGCLCs) which possess the potential to generate oocytes and sperms. Adipose-derived mesenchymal stromal cells (ASCs) are multipotent cells, having the capacity to differentiate into cell types such as adipocytes, osteocytes and chondrocytes. Since no information is available about the capability of female human ASCs (hASCs) to generate PGCLCs, we compared protocols to produce such cells from hASCs themselves or from hASC-derived iPSCs. The results showed that, providing pre-induction into a peri-gastrulating endoderm/mesoderm-like status, hASCs can generate PGCLCs. This process, however, shows a lower efficiency than when hASC-derived iPSCs are used as starting cells. Although hASCs possess multipotency and express mesodermal genes, direct induction into PGCLCs resulted less efficient.
在过去的二十年中，在从多能干细胞（如胚胎干细胞（ESCs）和诱导多能干细胞（iPSCs））中衍生哺乳动物生殖细胞方面已经取得了相当大的进展。这些多能干细胞通常首先被诱导进入原肠胚内胚层/中胚层样状态，然后被特化成为具有产生卵子和精子能力的所谓原始生殖细胞（PGCs），称为 PGCLCs。脂肪来源的间充质基质细胞（ASCs）是多能细胞，具有分化为脂肪细胞、成骨细胞和软骨细胞等细胞类型的能力。由于关于女性人类 ASCs（hASCs）产生 PGCLCs 的能力的信息尚不清楚，我们比较了从 hASCs 本身或从 hASC 衍生的 iPSCs 产生此类细胞的方案。结果表明，提供进入原肠胚内胚层/中胚层样状态的预诱导，hASCs 可以产生 PGCLCs。然而，与使用 hASC 衍生的 iPSCs 作为起始细胞相比，该过程的效率较低。尽管 hASCs 具有多能性并表达中胚层基因，但直接诱导成为 PGCLCs 的效率较低。
