Liu Haihui, Chen Mingtai, Liu Lulu, Ren Saisai, Cheng Panpan, Zhang Hao
1 Department of Graduate School, Jining Medical University , Jining, China .
2 Central Laboratory, Affiliated Hospital of Jining Medical University , Jining, China .
Cell Reprogram. 2018 Apr;20(2):127-134. doi: 10.1089/cell.2017.0063. Epub 2018 Mar 16.
Accumulating evidence indicates that mesenchymal stem cells (MSCs) have been widely used in tissue engineering and regenerative medicine due to their multilineage differentiation potentials. Recent studies show that germ-like cells can also be derived from stem cells, such as human umbilical cord MSCs and human bone marrow MSCs in vitro. However, whether human adipose-derived MSCs (hAD-MSCs) can be induced into germ-like cells has never been reported. In this study, we isolated hAD-MSCs and confirmed that their characteristics were in accordance with that of MSCs established before. Germ cell lineage differentiation was performed by 10 μM retinoic acid (RA) treatment for 21 days. RA induction led to slender spindles and tadpole-like changes of cell morphology, and the expression of germ cell-specific markers (Oct4, Piwil2, Itgb1, SSEA-1, and Stra8) presented significant upregulation in the RA treatment group according to the polymerase chain reaction and immunofluorescence results. We first demonstrated that hAD-MSCs can differentiate into germ-like cells in vitro, which will provide theoretical and experimental basis for the clinical application of hAD-MSCs in the treatment for infertility.
越来越多的证据表明，间充质干细胞（MSCs）因其多向分化潜能而被广泛应用于组织工程和再生医学。最近的研究表明，类生殖细胞也可以从干细胞中衍生而来，比如在体外从人脐带间充质干细胞和人骨髓间充质干细胞中获得。然而，人脂肪来源的间充质干细胞（hAD-MSCs）是否能被诱导分化为类生殖细胞尚未见报道。在本研究中，我们分离出hAD-MSCs，并证实其特性与之前建立的间充质干细胞特性相符。通过用10μM视黄酸（RA）处理21天来进行生殖细胞系分化。根据聚合酶链反应和免疫荧光结果，RA诱导导致细胞形态出现细长纺锤形和蝌蚪样变化，并且生殖细胞特异性标志物（Oct4、Piwil2、Itgb1、SSEA-1和Stra8）的表达在RA处理组中显著上调。我们首次证明hAD-MSCs在体外可分化为类生殖细胞，这将为hAD-MSCs在不孕症治疗中的临床应用提供理论和实验依据。
