一种用于细胞中次氯酸成像的线粒体靶向近红外荧光探针。
A mitochondria-targeting near-infrared fluorescent probe for imaging hypochlorous acid in cells.
作者信息
Zheng Aishan, Liu Han, Peng Chuanhao, Gao Xiaonan, Xu Kehua, Tang Bo
机构信息
College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Institute of Molecular and Nano Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Shandong Normal University, Jinan, 250014, PR China.
College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Institute of Molecular and Nano Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Shandong Normal University, Jinan, 250014, PR China.
出版信息
Talanta. 2021 May 1;226:122152. doi: 10.1016/j.talanta.2021.122152. Epub 2021 Jan 29.
Hypochlorous acid (HOCl), as an important reactive oxygen species (ROS), plays an important role in various pathological and physiological processes. Therefore, it is of great significance to identify and detect HOCl in organisms. Herein, a mitochondria-targeting near-Infrared fluorescent probe (CVS) has been designed and synthesized for sensing HOCl. Under simulated physiological conditions, CVS can be instantly oxidized by HOCl to CVSO and the fluorescence signal is significantly enhanced. Additionally, CVS shows high selectivity toward HOCl over other ROS and owns low detection limit (94.7 nM) for HOCl. Moreover, CVS can be successfully applied to image HOCl in mitochondria of the HepG2 cells. Thus, CVS is a powerful tool for sensing HOCl and provides a perspective method for further study of the physiological and pathological functions related to HOCl.
次氯酸（HOCl）作为一种重要的活性氧（ROS），在各种病理和生理过程中发挥着重要作用。因此，在生物体中识别和检测HOCl具有重要意义。在此，设计并合成了一种用于检测HOCl的线粒体靶向近红外荧光探针（CVS）。在模拟生理条件下，CVS可被HOCl瞬间氧化为CVSO，荧光信号显著增强。此外，CVS对HOCl的选择性高于其他ROS，对HOCl的检测限低（94.7 nM）。而且，CVS可成功应用于HepG2细胞线粒体中HOCl的成像。因此，CVS是检测HOCl的有力工具，为进一步研究与HOCl相关的生理和病理功能提供了一种有前景的方法。
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