Department of Laboratory Medicine, Ghent University Hospital, Ghent, Belgium.
Department of Diagnostic Sciences, Ghent University Hospital, Ghent, Belgium.
Clin Chem Lab Med. 2023 Jun 22;61(12):2186-2194. doi: 10.1515/cclm-2023-0327. Print 2023 Nov 27.
Well-standardized procedures in the pre-analytical phase of urine diagnostics is of utmost importance to obtain reliable results. We investigated the effect of different urine collection methods and the associated urine transfer tubes on urine test strip and particle results.
In total, 146 selected urine samples were subdivided into three different collection containers and subsequently transferred into its accompanying transfer tube (BD, Greiner, Sarstedt vacuum and Sarstedt aspiration). As reference, the original urine sample was directly measured on the analyser. Both chemical test strip analysis (Sysmex UC-3500) and fluorescence flow cytometry particle analysis (Sysmex UF-5000) were performed on all samples.
No statistically significant differences in test strip results were found between the studied transfer methods. On the contrary, transfer of urine samples to the secondary tubes affected their particle counts. Clinically significant reductions in counts of renal tubular epithelial cells and hyaline casts were observed using the BD and Greiner transfer tubes and in counts of pathological casts using the BD, Greiner and Sarstedt vacuum tubes.
The results of this study indicate that the use of urine transfer tubes may impact counts of fragile urine particles. Clinical laboratories need to be aware about the variation that urine collection methods can induce on urine particle counts.
尿液诊断分析前阶段的标准化程序非常重要，因为这是获得可靠结果的关键。本研究旨在探讨不同的尿液采集方法和相关的尿液转移管对尿液干化学试带和有形成分结果的影响。
共收集 146 份尿液标本，分为 3 种不同的采集容器，并分别转移至配套的转移管（BD、Greiner、Sarstedt 真空采尿管和 Sarstedt 负压采尿管）中。以原始尿液标本直接在分析仪上检测为参考。对所有标本均进行干化学试带分析（Sysmex UC-3500）和荧光流式细胞术有形成分分析（Sysmex UF-5000）。
研究的转移方法之间试带结果无统计学差异。相反，尿液样本转移至次级管会影响其有形成分计数。BD 和 Greiner 转移管中转运的尿液标本中肾小管上皮细胞和透明管型的计数显著减少，BD、Greiner 和 Sarstedt 真空采尿管中转运的尿液标本中病理性管型的计数也显著减少。
本研究结果表明，使用尿液转移管可能会影响易碎尿液有形成分的计数。临床实验室需要意识到尿液采集方法的变化可能会对尿液有形成分计数产生影响。
