Topcuoglu Canan, Sezer Sevilay, Kosem Arzu, Ercan Mujgan, Turhan Turan
a Biochemistry Department , Ankara Numune Training and Research Hospital , Ankara , Turkey.
b Department of Clinical Biochemistry, Faculty of Medicine , Bozok University , Yozgat , Turkey.
Scand J Clin Lab Invest. 2017 Dec;77(8):592-594. doi: 10.1080/00365513.2017.1371324. Epub 2017 Sep 4.
Urine collection systems with aspiration system for vacuum tubes are becoming increasingly common for urinalysis, especially for microscopic examination of the urine. In this study, we aimed to examine whether vacuum aspiration of the urine sample has any adverse effect on sediment analysis by comparing results from vacuum and non-vacuum urine tubes. The study included totally 213 urine samples obtained from inpatients and outpatients in our hospital. Urine samples were collected to containers with aspiration system for vacuum tubes. Each sample was aliquoted to both vacuum and non-vacuum urine tubes. Urinary sediment analysis was performed using manual microscope. Results were evaluated using chi-square test. Comparison of the sediment analysis results from vacuum and non-vacuum urine tubes showed that results were highly concordant for erythrocyte, leukocyte and epithelial cells (gamma values 1, 0.997, and 0.994, respectively; p < .001). Results were also concordant for urinary casts, crystals and yeast (kappa values 0.815, 0.945 and 1, respectively; p < .001). The results show that in urinary sediment analysis, vacuum aspiration has no adverse effect on the cellular components except on casts.
带有真空管抽吸系统的尿液收集系统在尿液分析中越来越普遍，尤其是在尿液显微镜检查方面。在本研究中，我们旨在通过比较真空管和非真空管尿液样本的结果，来检验尿液样本的真空抽吸对沉渣分析是否有任何不利影响。该研究共纳入了从我院住院患者和门诊患者中获取的213份尿液样本。将尿液样本收集到带有真空管抽吸系统的容器中。每个样本均被分装到真空管和非真空管中。使用手动显微镜进行尿沉渣分析。结果采用卡方检验进行评估。真空管和非真空管尿液样本的沉渣分析结果比较显示，红细胞、白细胞和上皮细胞的结果高度一致（γ值分别为1、0.997和0.994；p < .001）。尿管型、结晶和酵母的结果也一致（κ值分别为0.815、0.945和1；p < .001）。结果表明，在尿沉渣分析中，除管型外，真空抽吸对细胞成分没有不利影响。
