Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Guizhou Medical University, Guiyang, 550004, People's Republic of China.
Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education & Key Laboratory of Medical Molecular Biology of Guizhou Province, Guizhou Medical University, Guiyang, 550004, Guizhou, People's Republic of China.
Amino Acids. 2023 Aug;55(8):1063-1071. doi: 10.1007/s00726-023-03294-1. Epub 2023 Jun 21.
Diabetes Mellitus (DM) is one of the most important public health problems, and new antidiabetic drugs with fewer side effects are urgently needed. Here, we measured the antidiabetic effects of an antioxidant peptide (Ala-Phe-Tyr-Arg-Trp, AFYRW) from Tartary Buckwheat Albumin (TBA) in a high-fat diet/streptozotocin (HFD/STZ)-induced diabetic mouse model. The data showed that AFYRW suppressed hepatocyte steatosis and triglycerides while ameliorating insulin resistance in mice. Successively, the influence of AFYRW on aberrant protein glycosylation in diabetic mice was further investigated by lectin microarrays. The results suggested AFYRW could restore the expression of GalNAc, GalNAcα1-3Gal and GalNAcα1-3Galβ1-3/4Glc recognized by PTL-I, Siaα2-3Galβ1-4Glc(NAc)/Glc, Siaα2-3Gal, Siaα2-3 and Siaα2-3GalNAc recognized by MAL-II, terminating in GalNAcα/β1-3/6Gal recognized by WFA and αGalNAc, αGal, anti-A and B recognized by GSI-I to normal levels in the pancreas of HFD-STZ-induced diabetic mice. This work may provide new targets for the future discovery of potential biomarkers to evaluate the efficacy of food-derived antidiabetic drugs based on precise alterations of glycopatterns in DM.
糖尿病（DM）是最重要的公共卫生问题之一，迫切需要具有更少副作用的新型抗糖尿病药物。在这里，我们在高脂肪饮食/链脲佐菌素（HFD/STZ）诱导的糖尿病小鼠模型中测量了来自苦荞白蛋白（TBA）的抗氧化肽（Ala-Phe-Tyr-Arg-Trp，AFYRW）的抗糖尿病作用。数据显示，AFYRW 抑制肝细胞脂肪变性和甘油三酯，同时改善小鼠胰岛素抵抗。随后，通过凝集素微阵列进一步研究了 AFYRW 对糖尿病小鼠异常蛋白糖基化的影响。结果表明，AFYRW 可以恢复 PTL-I 识别的 GalNAc、GalNAcα1-3Gal 和 GalNAcα1-3Galβ1-3/4Glc、Siaα2-3Galβ1-4Glc(NAc)/Glc、Siaα2-3Gal、Siaα2-3 和 Siaα2-3GalNAc 的表达，终止于 HFD-STZ 诱导的糖尿病小鼠胰腺中 GalNAcα/β1-3/6Gal 识别的 WFA 和αGalNAc、αGal、抗-A 和 B 的表达水平正常。这项工作可能为未来发现基于 DM 中糖基化模式的精确改变来评估食源抗糖尿病药物疗效的潜在生物标志物提供新的靶点。
