Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou 571158, China.
J Agric Food Chem. 2023 Jul 5;71(26):10107-10123. doi: 10.1021/acs.jafc.3c00122. Epub 2023 Jun 21.
Lysine lactylation (kla) as a post-translational modification (PTM) is linked to chromatin remodeling and gene transcription. Nitrogen stress can induce triacylglyceride (TAG) accumulation in most microalgae. To reveal lipid synthesis from another layer, the 4D label-free proteome method was used to track lactylation modifications and protein expression profiles after 3 days of nitrogen deprivation in to analyze the function and prevalence of lactylation. Under nitrogen deprivation (ND) and nitrogen repletion (NR) cultivation conditions, we mapped the proteome and lactylome of proliferating microalgal cells. The results showed that there were 5203 proteins identified and 1245 proteins differentially expressed; among them, 664 were upregulated and 581 were downregulated. The strain also has a total of 868 lactylation sites, which were found on 379 proteins. The lactylated proteins were linked to biological activities as diverse as lipid metabolism, carbon fixation, photosynthesis, glycolysis, and the tricarboxylic acid (TCA) cycle and were found in various subcellular compartments. Under ND, 414 specific lactylation sites were found in 252 proteins. More interestingly, under ND vs NR, lactylation showed a significantly upregulated trend. This study provides a foundation for the future epigenetic enhancement of microalgal cells by providing the first dataset of lactylome in .
赖氨酸酰化（kla）作为一种翻译后修饰（PTM），与染色质重塑和基因转录有关。氮胁迫可以诱导大多数微藻中三酰基甘油（TAG）的积累。为了从另一个层面揭示脂质合成，采用 4D 无标记蛋白质组学方法跟踪氮饥饿 3 天后 的酰化修饰和蛋白质表达谱，以分析酰化的功能和普遍性。在氮饥饿（ND）和氮补充（NR）培养条件下，我们绘制了增殖微藻细胞的蛋白质组和酰基组图谱。结果表明，鉴定出 5203 种蛋白质和 1245 种差异表达蛋白质；其中 664 种上调，581 种下调。该 株总共也有 868 个酰化位点，分布在 379 种蛋白质上。酰化蛋白与脂质代谢、碳固定、光合作用、糖酵解和三羧酸（TCA）循环等多种生物活性有关，存在于各种亚细胞区室中。在 ND 条件下，在 252 种蛋白质中发现了 414 个特异性酰化位点。更有趣的是，与 NR 相比，ND 下的酰化呈现出明显上调的趋势。本研究为未来通过表观遗传增强微藻细胞提供了基础，首次提供了 中的酰基组数据集。
