Feng Konglong, Duan Yashan, Zhang Huiting, Xiao Jie, Ho Chi-Tang, Huang Qingrong, Cao Yong
Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Sciences, South China Agricultural University, Guangzhou, Guangdong 510642, China.
Department of Food Science, Rutgers University, 65 Dudley Road, New Brunswick, New Jersey 08901, USA.
Food Funct. 2023 Jul 3;14(13):6212-6225. doi: 10.1039/d3fo00543g.
Lipid-based delivery systems are commonly used to encapsulate hydrophobic bioactive compounds for enhancing their bioaccessibility and bioavailability, especially for triacylglycerol (TAG) oil-based delivery systems. However, studies on the development of 1,3-diacylglycerol (DAG) oil-based delivery systems are rather limited. Herein, the influence of 1,3-DAG oil as a carrier oil on the properties of nanoemulsions and the bioaccessibility of encapsulated hydrophobic nobiletin (NOB) were investigated. High-purity 1,3-DAG (over 93% pure) was prepared by a combination of enzymatic esterification and ethanol crystallization. 1,3-DAG oil as a carrier oil could be used to formulate nanoemulsions with smaller droplet size, narrower size distribution and similar stability compared to TAG oil. Importantly, 1,3-DAG oil could efficiently encapsulate high-loading NOB (1.45 mg g) in nanoemulsions and significantly improve the bioaccessibility of NOB (above 80%), which is attributable to its massive lipolysis and higher encapsulation capacity than TAG oil. Moreover, the addition of the 1,3-DAG component in TAG oil significantly improved the properties of nanoemulsions and the loading and bioaccessibility of NOB, especially as the 1,3-DAG content was not less than 50%. The structure of lipids (DAG TAG) influenced the nanoemulsion properties and the bioaccessibility of encapsulated NOB. Based on the good properties of 1,3-DAG oil coupled with its health benefits, 1,3-DAG oil-based nanoemulsion delivery systems have great prospects for improving and extending emulsion properties and bioactivity as well as bioaccessibility enhancement.
基于脂质的递送系统通常用于包封疏水性生物活性化合物，以提高其生物可及性和生物利用度，特别是对于基于三酰甘油（TAG）油的递送系统。然而，关于基于1,3-二酰甘油（DAG）油的递送系统的开发研究相当有限。在此，研究了1,3-DAG油作为载体油对纳米乳液性质以及包封的疏水性诺米林（NOB）生物可及性的影响。通过酶促酯化和乙醇结晶相结合的方法制备了高纯度的1,3-DAG（纯度超过93%）。与TAG油相比，1,3-DAG油作为载体油可用于制备粒径更小、粒径分布更窄且稳定性相似的纳米乳液。重要的是，1,3-DAG油能够在纳米乳液中高效包封高负载量的NOB（1.45 mg/g），并显著提高NOB的生物可及性（超过80%），这归因于其大量的脂解作用以及比TAG油更高的包封能力。此外，在TAG油中添加1,3-DAG组分显著改善了纳米乳液的性质以及NOB的负载量和生物可及性，特别是当1,3-DAG含量不低于50%时。脂质结构（DAG与TAG）影响了纳米乳液的性质以及包封的NOB的生物可及性。基于1,3-DAG油的良好性质及其对健康的益处，基于1,3-DAG油的纳米乳液递送系统在改善和扩展乳液性质、生物活性以及提高生物可及性方面具有广阔的前景。
