Amet Y, Abalain J H, di Stefano S, Daniel J Y, Tea K, Floch H H, Robel P
J Endocrinol. 1986 Jun;109(3):299-306. doi: 10.1677/joe.0.1090299.
An exchange assay for androgen receptors in the quail uropygial gland using [3H]mibolerone was established. The most efficient exchange conditions were 3 days of incubation at 15 degrees C. Under these conditions, androgen receptors were stable in the presence of sodium molybdate, and the exchange of [3H]mibolerone with endogenous testosterone bound to cytosolic or nuclear androgen receptors was maximal. Less than 5% of [3H]mibolerone-binding sites occurred in the extracted nuclear pellets. Using this exchange technique, it was shown that androgen receptors in the uropygial gland of photostimulated male quail or castrated quail treated with testosterone were activated and that their concentrations in both cytosolic and nuclear fractions were increased. These results confirm the androgen dependency of the quail uropygial gland, and show that it is an organ which can be used as a model for the study of androgen action in sebaceous glands.
建立了一种使用[3H]美勃酮对鹌鹑尾脂腺雄激素受体进行交换分析的方法。最有效的交换条件是在15℃孵育3天。在这些条件下，雄激素受体在钼酸钠存在时稳定，并且[3H]美勃酮与结合到胞质或核雄激素受体上的内源性睾酮的交换达到最大值。少于5%的[3H]美勃酮结合位点出现在提取的核沉淀中。使用这种交换技术表明，光照刺激的雄性鹌鹑或用睾酮处理的去势鹌鹑的尾脂腺中的雄激素受体被激活，并且它们在胞质和核部分中的浓度都增加了。这些结果证实了鹌鹑尾脂腺对雄激素的依赖性，并表明它是一种可用于研究皮脂腺中雄激素作用的模型器官。
