Department of Organ Anatomy and Nanomedicine, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan; Core Clusters for Research Initiatives of Yamaguchi University, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan.
Department of Organ Anatomy and Nanomedicine, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan.
Colloids Surf B Biointerfaces. 2023 Aug;228:113397. doi: 10.1016/j.colsurfb.2023.113397. Epub 2023 Jun 14.
We investigated the distribution of intravenously administered thiol-organosilica particle (thiol-OS) in the spleen to evaluate their size effect in mice. A single administration of particles of thiol-OS containing rhodamine B (Rh) (90, 280, 340, 450, 630, 1110, 1670, and 3030 nm in diameter) was performed. After 24 h, we conducted a combination analysis using histological studies by fluorescent microscopy and quantitative inductively coupled plasma optical emission spectrometry (ICP-OES), which revealed no clear correlation between the particle size and spleen uptake of particle weight and number per tissue weight, and the injection dose. Moreover, Rh with 450 nm diameter (Rh450) showed the highest uptake, and Rh with 340 nm diameter (Rh340) showed the lowest uptake. Histologically, large fluorescent areas in the marginal zone (MZ) and red pulp (RP) of the spleen were observed for all particle sizes, but less in the follicle of white pulp. Using combination analysis using the particle weights of ICP-OES and the fluorescent area, we compared the distributions of each particle in each region. Rh450 had the largest accumulated weight in the MZ and RP. Particles larger than Rh450 showed negative correlations between their sizes and accumulated weight in the MZ and RP. Simultaneous dual administration of particles using Rhs and thiol-OS containing fluorescein (90 nm in diameter) showed the size-dependent difference in cellular distribution and intracellular localization. Immunohistochemical staining against macrophage markers, CD169, and F4/80 showed various colocalization patterns with macrophages that uptook particles, indicating differences in particle uptake in each macrophage may have novel significance.
我们研究了静脉注射的巯基有机硅纳米粒子（thiol-OS）在脾脏中的分布，以评估其在小鼠中的粒径效应。单次给予含罗丹明 B（Rh）的巯基-OS 纳米粒子（直径分别为 90、280、340、450、630、1110、1670 和 3030nm）。24 小时后，我们结合荧光显微镜组织学研究和定量电感耦合等离子体光学发射光谱（ICP-OES）进行了组合分析，结果显示，粒径与脾脏对粒子重量和单位组织重量的摄取量以及注射剂量之间没有明显的相关性。此外，直径为 450nm 的 Rh（Rh450）摄取量最高，直径为 340nm 的 Rh（Rh340）摄取量最低。组织学上，观察到所有粒径的脾脏边缘区（MZ）和红髓（RP）中均有较大的荧光区域，但在白髓的滤泡中则较少。使用 ICP-OES 的粒子重量和荧光面积的组合分析，我们比较了每个粒子在每个区域的分布。Rh450 在 MZ 和 RP 中的累积重量最大。大于 Rh450 的粒子在 MZ 和 RP 中的累积重量与其粒径之间呈负相关。同时给予 Rh 和含有荧光素（直径 90nm）的巯基-OS 粒子，显示出细胞分布和细胞内定位的粒径依赖性差异。针对巨噬细胞标记物 CD169 和 F4/80 的免疫组织化学染色显示了与吞噬粒子的巨噬细胞的各种共定位模式，表明每个巨噬细胞对粒子的摄取存在差异，这可能具有新的意义。
