Department of Anatomy and Cell Biology, The University of Tokushima Graduate School, Tokushima, Japan.
Nanomedicine. 2013 Feb;9(2):274-83. doi: 10.1016/j.nano.2012.05.018. Epub 2012 Jun 12.
Fluorescent thiol-organosilica nanoparticles with 100 nm diameter (F-thiol-OS-100) were applied for time-lapse fluorescence imaging. The evaluation of F-thiol-OS-100 for quantitative analysis demonstrated great advantages as compared with quantum dots and organic fluorescent dye. Time-lapse fluorescence imaging of mouse peritoneal macrophages using F-thiol-OS-100 clearly demonstrated cellular uptake, and single cell analysis showed various patterns of uptake kinetics that could be quantitatively evaluated. We also performed quantitative analysis of endosomal uptake and movements in single cells. A correlation between morphologic findings and endosomal uptake and movement over time was also observed and analyzed quantitatively. The F-thiol-OS-100 showed high potential as a new fluorescence marker for time-lapse fluorescence imaging and quantitative single cell functional analysis for nanomedicine development.
In this study the authors report on 100 nm thiol-organosilica nanoparticles as time-lapse flurescent markers. F-thiol-OS-100 proved to be superior to quantum dots and organic flurescent dyes, and enabled quantitative single cell functional analysis.
100nm 直径的荧光硫醇有机硅纳米粒子（F-thiol-OS-100）被应用于延时荧光成像。与量子点和有机荧光染料相比，F-thiol-OS-100 用于定量分析具有显著优势。使用 F-thiol-OS-100 对小鼠腹腔巨噬细胞进行延时荧光成像，清楚地显示了细胞摄取，单细胞分析显示了多种摄取动力学模式，可进行定量评估。我们还对细胞内体摄取和运动进行了定量分析。还观察到并定量分析了形态学发现与内体摄取和随时间推移的运动之间的相关性。F-thiol-OS-100 有望成为一种新的荧光标记物，用于延时荧光成像和纳米医学发展的定量单细胞功能分析。
在这项研究中，作者报告了 100nm 硫醇有机硅纳米粒子作为延时荧光标记物。F-thiol-OS-100 被证明优于量子点和有机荧光染料，并且能够进行定量的单细胞功能分析。
