Walker R A, Day S J
J Pathol. 1986 Jun;149(2):113-20. doi: 10.1002/path.1711490206.
Monoclonal antibodies directed against O(H) blood group antigen and 3-fucosyl-N-acetyllactosamine have been used to investigate the incidence and significance of these type 2 blood group chains in non-malignant and malignant human breast tissue. The findings have been compared with those obtained with the fucose-binding lectins, Ulex europeus I and Lotus tetragonolobus. The expression of O(H) antigen in non-malignant breast is heterogeneous and shows no relationship to blood group status. Its expression in carcinomas shows no relationship to prognostic parameters such as differentiation and node status, whereas loss of Ulex europeus I binding is related to metastasis. The presence of 3-fucosyl-N-acetyllactosamine is limited in non-malignant breast, apart from pregnancy, but in carcinomas shows a relationship to differentiation, a feature not seen with fucose-binding lectins. It is apparent that to obtain the maximum amount of information about the modifications occurring in fuco-substances that appear to be of significance in breast malignancy, it is important to use monospecific probes as well as reagents with broader specificities, such as lectins.
针对O(H)血型抗原和3-岩藻糖基-N-乙酰乳糖胺的单克隆抗体已被用于研究这些2型血型链在非恶性和恶性人乳腺组织中的发生率及意义。研究结果已与使用岩藻糖结合凝集素（欧洲荆豆I和四角百脉根）所获得的结果进行了比较。O(H)抗原在非恶性乳腺中的表达是异质性的，且与血型状态无关。其在癌组织中的表达与分化和淋巴结状态等预后参数无关，而欧洲荆豆I结合的丧失与转移有关。除妊娠外，3-岩藻糖基-N-乙酰乳糖胺在非恶性乳腺中的存在有限，但在癌组织中与分化有关，这是岩藻糖结合凝集素未显示的特征。显然，为了获取关于在乳腺恶性肿瘤中似乎具有重要意义的岩藻糖物质修饰的最大信息量，使用单特异性探针以及具有更广泛特异性的试剂（如凝集素）非常重要。
