Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China.
Anal Chem. 2022 Jan 18;94(2):1221-1229. doi: 10.1021/acs.analchem.1c04413. Epub 2021 Dec 29.
In this work, a polarization-resolved electrochemiluminescence (ECL) sensor for microRNA-155 (miRNA-155) detection has been constructed based on the surface plasmon coupling effect. In the sensing system, nitrogen dots (N dots) were employed as ECL emitters. As a surface-enhanced structure, a gold nanorod vertical array was constructed on the electrode surface by the volatilization-induced self-assembly. The coupling of the adjacent gold nanorods in the array can generate significant local electromagnetic fields. Due to the anisotropy of gold nanorods and the hot spot effect of the vertical array, the ECL signal of N dots was greatly improved at a specific polarization angle. In addition, the catalytic hairpin self-assembly strategy was used to amplify the nucleic acid analyte signal. As a result, the polarization-resolved ECL sensor can detect miRNA-155 sensitively, which is related to triple-negative breast cancer.
在这项工作中，基于表面等离激元耦合效应，构建了一种用于检测 microRNA-155（miRNA-155）的偏振分辨电化学发光（ECL）传感器。在传感系统中，氮点（N 点）被用作 ECL 发射器。作为表面增强结构，通过蒸发诱导自组装在电极表面上构建了金纳米棒垂直阵列。阵列中相邻金纳米棒的耦合可以产生显著的局部电磁场。由于金纳米棒的各向异性和垂直阵列的热点效应，N 点的 ECL 信号在特定偏振角下得到了极大的增强。此外，还利用催化发夹自组装策略来放大核酸分析物信号。因此，偏振分辨 ECL 传感器可以灵敏地检测与三阴性乳腺癌相关的 miRNA-155。
