Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou, Guangdong, P.R. China.
Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, Guangdong, P.R. China.
Food Funct. 2023 Jul 3;14(13):6248-6261. doi: 10.1039/d3fo01095c.
This study investigated the preventive effect of 5-demethylnobiletin (5DN), a natural polymethoxyflavone found mainly in citrus fruits, on dextran sulfate sodium (DSS)-induced colitis in mice and explored its potential mechanisms. Our results indicated that dietary 5DN (0.05% w/w in diet) could alleviate colitis symptoms in DSS-treated mice by preventing body weight loss, reducing the disease activity index, decreasing the colon weight to colon length ratio, and lessening colon tissue damage. Additionally, 5DN inhibited the inflammatory response in colitis mice through decreasing the production of inflammatory cytokines. Immunohistochemical analysis revealed that 5DN could reverse the DSS-induced decrease in the expression of claudin-1 and ZO-1 to improve the intestinal barrier function. Furthermore, 5DN altered gut microbiota dysbiosis in DSS-treated mice up-regulating the level of probiotics () and down-regulating the level of pathogenic bacteria (, , and ). Taken together, these data provided a solid scientific basis for utilizing 5DN as a therapeutic candidate in colitis and related diseases.
本研究旨在探讨 5-去甲川陈皮素（5DN）对葡聚糖硫酸钠（DSS）诱导的结肠炎小鼠的预防作用，并探讨其潜在机制。5DN 是一种主要存在于柑橘类水果中的天然多甲氧基黄酮。我们的研究结果表明，饮食中添加 5DN（饮食中 0.05%w/w）可通过预防体重减轻、降低疾病活动指数、降低结肠重量与结肠长度比以及减轻结肠组织损伤，从而减轻 DSS 处理的小鼠的结肠炎症状。此外，5DN 通过减少炎症细胞因子的产生抑制了结肠炎小鼠的炎症反应。免疫组化分析显示，5DN 可逆转 DSS 诱导的 Claudin-1 和 ZO-1 表达降低，从而改善肠道屏障功能。此外，5DN 改变了 DSS 处理的小鼠肠道微生物失调，上调了益生菌（）水平，下调了致病菌（、、和）水平。综上所述，这些数据为将 5DN 作为结肠炎和相关疾病的治疗候选药物提供了坚实的科学依据。
