Al-Jwary Enas Talb, Al-Saleem Niam Riyadh
Department of Pedodontic, Orthodontic and Preventive Dentistry, Collage of Dentistry, University of Mosul, Iraq.
J Orthod Sci. 2023 Mar 18;12:16. doi: 10.4103/jos.jos_109_22. eCollection 2023.
This study aimed to evaluate the effect of the local injection of injectable platelet-rich fibrin (i-PRF) on the eruption rate of the teeth and evaluation of the effect of i-PRF on the number of odontoblast, cementoblast, osteoblast, osteocyte, and osteoclast cells.
The samples consisted of 20 male albino rabbits and were divided randomly into the control group (5 rabbits) and i-PRF groups (15 rabbits) sub-divided into week 1, week 2, and week 3 sub-groups. The labial surface of lower right incisors was marked by drilling a hole at the level of the free gingival margin in the mid-line of each tooth with a small round bur, and the eruption rates were measured by measuring the distance from the most apical point of the free gingival margin and the center of the hole with a digital caliper. i-PRF was prepared by centrifuging autologous blood of each rabbit at 1000 rpm for 5 minutes; then the i-PRF layer was collected. The control group received no treatment, whereas i-PRF groups received i-PRF in the following manner: For week 1 groups, a single dose was given at 0 day; for week 2 groups, double doses were given at 0 and 7 days; and for week 3 groups, three doses were given at 0, 7, and 14 days. At the end of experiments for each group, animal scarification was performed, and histological steps were performed.
There was a significant increase in the rate of tooth eruption in i-PRF groups when compared to the control group, and the eruption rate was increased in 107.3%, 144.7%, and 167.5% for week 1, week 2, and week 3, respectively. Concerning the histological finding, the i-PRF groups gave rise to a higher number of odontoblast, cementoblast, osteoblast, osteocyte, and osteoclast cells with a significant difference when compared to the control groups ( ≤ 0.05).
Applying i-PRF significantly increased the rate of teeth eruption at all-time intervals. Platelet-rich fibrin injection can be an effective method for acceleration of teeth eruption.
本研究旨在评估局部注射可注射富血小板纤维蛋白（i-PRF）对牙齿萌出率的影响，并评估i-PRF对成牙本质细胞、成牙骨质细胞、成骨细胞、骨细胞和破骨细胞数量的影响。
样本包括20只雄性白化兔，随机分为对照组（5只兔）和i-PRF组（15只兔），i-PRF组再分为第1周、第2周和第3周亚组。用小圆钻在每颗右下切牙游离龈缘水平的中线处钻孔标记唇面，用数字卡尺测量游离龈缘最根尖点与孔中心的距离来测量萌出率。通过将每只兔子的自体血以1000 rpm离心5分钟制备i-PRF；然后收集i-PRF层。对照组不接受治疗，而i-PRF组按以下方式接受i-PRF：第1周组在第0天给予单剂量；第2周组在第0天和第7天给予双剂量；第3周组在第0天、第7天和第14天给予三剂量。每组实验结束时，进行动物放血，并进行组织学步骤。
与对照组相比，i-PRF组的牙齿萌出率显著增加，第第1周、第2周和第3周的萌出率分别提高了107.3%、144.7%和167.5%。关于组织学发现，与对照组相比，i-PRF组产生了更多的成牙本质细胞、成牙骨质细胞、成骨细胞、骨细胞和破骨细胞，差异有统计学意义（P≤0.05）。
应用i-PRF在所有时间间隔均显著提高了牙齿萌出率。注射富血小板纤维蛋白可以是加速牙齿萌出的有效方法。
