Department of Ophthalmology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
Biomarkers. 2023 Sep;28(6):531-537. doi: 10.1080/1354750X.2023.2229532. Epub 2023 Jul 11.
Contact lens discomfort (CLD) acts as a challenging problem, and the associated conjunctival microbiome changes were unclear.
Conjunctival sac swab samples were collected from 12 eyes of nonwearers (NW), 12 eyes of asymptomatic contact lens (ACL) wearers, and 11 eyes of CLD. The V3-V4 region of the 16S rRNA gene sequencing was used to investigate differences among three groups.
No differences in alpha diversity were observed among the three groups. The beta diversity showed a distinct microbiome composition between ACL and CLD group ( = 0.018) with principal coordinate analysis. The relative abundance of was significantly higher in CLD (48.18%) than in ACL (13.21%) group ( = 0.018). The abundance of in patients with ACL (0.05%) or with CLD (0.02%) were significantly lower than that in the NW (1.27%) group ( = 0.024, 0.028, respectively). Moreover, the abundance of was positively correlated with the OSDI scores in CLD patients (r = 0.817, < 0. 01, Spearman).
Patients with CLD have various degrees of bacterial microbiota imbalance in the conjunctival sac, compared with NW and ACL groups.
may serve as a potential biomarker for the CLD patients.
隐形眼镜不适（CLD）是一个具有挑战性的问题，其相关的结膜微生物组变化尚不清楚。
从 12 名非佩戴者（NW）、12 名无症状隐形眼镜（ACL）佩戴者和 11 名 CLD 患者的 12 只眼中采集结膜囊拭子样本。使用 16S rRNA 基因测序的 V3-V4 区来研究三组之间的差异。
三组之间的 alpha 多样性没有差异。β多样性显示 ACL 和 CLD 组之间的微生物组组成明显不同（=0.018），主坐标分析。CLD 组中（48.18%）的相对丰度显著高于 ACL 组（13.21%）（=0.018）。ACL（0.05%）或 CLD（0.02%）患者中 丰度明显低于 NW（1.27%）组（=0.024，0.028）。此外，CLD 患者的 OSDI 评分与 丰度呈正相关（r=0.817，p<0.01，Spearman）。
与 NW 和 ACL 组相比，CLD 患者的结膜囊中存在不同程度的细菌微生物群落失衡。
可能是 CLD 患者的潜在生物标志物。
