Zhang Haikun, Zhao Fuxin, Hutchinson Diane S, Sun Wenfeng, Ajami Nadim J, Lai Shujuan, Wong Matthew C, Petrosino Joseph F, Fang Jianhuo, Jiang Jun, Chen Wei, Reinach Peter S, Qu Jia, Zeng Changqing, Zhang Dake, Zhou Xiangtian
Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China 2University of Chinese Academy of Sciences, Beijing, China.
School of Optometry and Ophthalmology and Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China 4State Key Laboratory Cultivation Base and Key Laboratory of Vision Science, Ministry of Health P. R. China and Zhejiang Provincial Key Laboratory of Ophthalmology and Optometry, Wenzhou, Zhejiang, China.
Invest Ophthalmol Vis Sci. 2017 Jan 1;58(1):128-136. doi: 10.1167/iovs.16-20231.
Usage of different types of contact lenses is associated with increased risk of sight-threatening complications. Changes in the ocular microbiome caused by contact lens wear are suggested to affect infection development in those individuals. To address this question, this study compares conjunctival microbial communities in contact lens wearers with those in noncontact lens wearers.
Paired-end sequencing of the V3 region of the 16S rRNA gene was used to characterize the bacterial communities on the conjunctival surfaces of contact lens wearers and nonwearers.
No differences in microbial diversity were detected between contact lens wearers and nonwearers. Nevertheless, some slight microbe variability was evident between these two different groups. Bacillus, Tatumella and Lactobacillus abundance was less in orthokeratology lens (OKL) wearers than in nonwearers. In soft contact lenses (SCL) wearers, Delftia abundance decreased whereas Elizabethkingia levels increased. The difference in the SCL and nonwearer group was smaller than that in the OKL group. Variations in the conjunctival taxonomic composition between SCL wearers were larger than those in other groups. Sex differences in the conjunctival microbiota makeup were only evident among nonwearers.
Even though there were slight percentage changes between contact lens wearers and nonwearers in some microbes, there were no differences in their diversity. On the other hand, contact lens usage might cause relative abundance of some taxa to change. Our results will help assess whether or not conjunctival microbiome changes caused by contact lens wear affect infection risk.
使用不同类型的隐形眼镜与视力威胁性并发症风险增加相关。有研究表明，隐形眼镜佩戴引起的眼部微生物群变化会影响这些个体的感染发展。为解决这一问题，本研究比较了隐形眼镜佩戴者和非隐形眼镜佩戴者的结膜微生物群落。
采用16S rRNA基因V3区域的双端测序来表征隐形眼镜佩戴者和非佩戴者结膜表面的细菌群落。
在隐形眼镜佩戴者和非佩戴者之间未检测到微生物多样性的差异。然而，这两组之间存在一些轻微的微生物变异性。角膜塑形镜（OKL）佩戴者中芽孢杆菌、塔特姆菌和乳杆菌的丰度低于非佩戴者。在软性隐形眼镜（SCL）佩戴者中，代尔夫特菌丰度降低，而伊丽莎白菌水平升高。SCL组与非佩戴者组之间的差异小于OKL组。SCL佩戴者之间结膜分类组成的差异大于其他组。结膜微生物群组成的性别差异仅在非佩戴者中明显。
尽管隐形眼镜佩戴者和非佩戴者之间某些微生物的百分比有轻微变化，但它们的多样性没有差异。另一方面，使用隐形眼镜可能会导致一些分类群的相对丰度发生变化。我们的结果将有助于评估隐形眼镜佩戴引起的结膜微生物群变化是否会影响感染风险。
