Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA.
Rocky Mountain Veterinary Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA.
J Infect Dis. 2023 Nov 13;228(Suppl 7):S548-S553. doi: 10.1093/infdis/jiad226.
Type I interferon receptor knockout (IFNAR-/-) mice are not able to generate a complete innate immune response; therefore, these mice are often considered to assess the pathogenicity of emerging viruses. We infected IFNAR-/- mice with a low or high dose of Lloviu virus (LLOV) or Bombali virus (BOMV) by the intranasal (IN) or intraperitoneal (IP) route and compared virus loads at early and late time points after infection. No signs of disease and no viral RNA were detected after IN infection regardless of LLOV dose. In contrast, IP infections resulted in increased viral loads in the high-dose LLOV and BOMV groups at the early time point. The low-dose LLOV and BOMV groups achieved higher viral loads at the late time point. However, there was 100% survival in all groups and no signs of disease. In conclusion, our results indicate a limited value of the IFNAR-/- mouse model for investigation of the pathogenicity of LLOV and BOMV.
I 型干扰素受体敲除（IFNAR-/-）小鼠不能产生完整的先天免疫反应；因此，这些小鼠常被用于评估新兴病毒的致病性。我们通过鼻腔内（IN）或腹腔内（IP）途径感染 IFNAR-/- 小鼠低剂量或高剂量的 Lloviu 病毒（LLOV）或 Bombali 病毒（BOMV），并比较感染后早期和晚期的病毒载量。无论 LLOV 剂量如何，IN 感染后均未出现疾病迹象和检测到病毒 RNA。相比之下，IP 感染会导致高剂量 LLOV 和 BOMV 组在早期时间点的病毒载量增加。低剂量 LLOV 和 BOMV 组在晚期时间点达到更高的病毒载量。然而，所有组均 100%存活，没有疾病迹象。总之，我们的结果表明，IFNAR-/- 小鼠模型对于研究 LLOV 和 BOMV 的致病性的价值有限。
