Dybvig K, Sladek T L, Maniloff J
J Virol. 1986 Sep;59(3):584-90. doi: 10.1128/JVI.59.3.584-590.1986.
We isolated two spontaneous variants of mycoplasma virus L2. Both variants, designated L2ins1 and L2ins2, contained a 3.1-kilobase-pair (kbp) insertion in the 11.8-kbp wild-type L2 genome. The insert DNA was shown to be derived from two noncontiguous regions of the L2 genome, and L2ins1 and L2ins2 differed only in the location of the 3.1-kbp insertion. We also isolated L2 miniviruses from serial passages of L2, L2ins1, and L2ins2 viruses. Miniviruses contained circular DNA molecules of 3.1 kbp or multimers of 3.1 kbp. Minivirus 3.1-kbp DNAs had the same sequences as the 3.1-kbp insert DNAs found in L2ins1 and L2ins2 viruses. Miniviruses were not infectious and interfered with the growth of L2, L2ins1, and L2ins2 viruses; hence, L2 miniviruses appeared to be defective interfering particles.
我们分离出了支原体病毒L2的两个自发变体。这两个变体，分别命名为L2ins1和L2ins2，在11.8千碱基对（kbp）的野生型L2基因组中都含有一个3.1千碱基对（kbp）的插入片段。插入的DNA显示来自L2基因组的两个不连续区域，并且L2ins1和L2ins2仅在3.1千碱基对插入片段的位置上有所不同。我们还从L2、L2ins1和L2ins2病毒的连续传代中分离出了L2微小病毒。微小病毒含有3.1千碱基对的环状DNA分子或3.1千碱基对的多聚体。微小病毒的3.1千碱基对DNA与在L2ins1和L2ins2病毒中发现的3.1千碱基对插入DNA具有相同的序列。微小病毒没有传染性，并干扰L2、L2ins1和L2ins2病毒的生长；因此，L2微小病毒似乎是缺陷干扰颗粒。
