支原体病毒DNA复制起始点和终点的定位

Mapping of mycoplasma virus DNA replication origins and termini.

作者信息

Poddar S K, Maniloff J

出版信息

J Virol. 1987 Jun;61(6):1909-12. doi: 10.1128/JVI.61.6.1909-1912.1987.

DOI:10.1128/JVI.61.6.1909-1912.1987

PMID:3573152

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC254197/

Abstract

A pulse-labeling protocol has been used to study DNA replication and map replication origins and termini in mycoplasma viruses L2 and L2ins1. The L2 genome is circular, double-stranded DNA of 11.63 kilobase pairs (kb), and the 14.89-kb L2ins1 genome is L2 DNA containing a 3.26-kb insertion. The data show that DNA replication is bidirectional from two origins in L2 and three origins in L2ins1. The extra origin in L2ins1 arises from the fact that one of the L2 origins is in one of the sequences that have been shown to be duplicated and transposed in the generation of L2ins1 from L2.

摘要

一种脉冲标记方案已被用于研究支原体病毒L2和L2ins1中的DNA复制以及绘制复制起点和终点。L2基因组是一个由11.63千碱基对（kb）组成的环状双链DNA，而14.89 kb的L2ins1基因组是包含一个3.26 kb插入片段的L2 DNA。数据表明，L2中的DNA复制从两个起点双向进行，而L2ins1中的DNA复制从三个起点双向进行。L2ins1中的额外起点源于这样一个事实，即L2的一个起点位于在从L2产生L2ins1的过程中已被证明发生了复制和转座的序列之中。