Sladek T L, Maniloff J
J Virol. 1985 Jan;53(1):25-31. doi: 10.1128/JVI.53.1.25-31.1985.
Double-stranded DNA from mycoplasma virus L2 can transfect Acholeplasma laidlawii cells in the presence of polyethylene glycol (T. L. Sladek and J. Maniloff, J. Bacteriol. 155:734-741, 1983). We report here that both single-stranded DNA and double-stranded replicative form DNA, from the single-stranded DNA mycoplasma virus L51, are also infectious in this system. For both DNAs transfection frequencies were in the range of 10(-8) transfectants per DNA molecule and 10(-3) transfectants per CFU. An unexpected finding was that both DNAs could transfect A. laidlawii strain REP-, a variant which is a nonpermissive host for single-stranded DNA mycoplasma viruses due to a block in viral DNA replication (Nowak et al., J. Bacteriol. 127:832-836, 1976). The number of viruses produced by transfected REP- cells was comparable to the number produced by both transfected and infected wild-type cells. Therefore, transfected L51 DNAs are able to bypass the replication block in REP- cells that occurs when these cells are infected by L51 virions.
在聚乙二醇存在的情况下，来自支原体病毒L2的双链DNA能够转染莱氏无胆甾原体细胞（T. L. 斯莱德克和J. 马尼洛夫，《细菌学杂志》155:734 - 741, 1983）。我们在此报告，来自单链DNA支原体病毒L51的单链DNA和双链复制型DNA在该系统中也具有感染性。对于这两种DNA，转染频率均在每DNA分子10^(-8)个转染子以及每CFU 10^(-3)个转染子的范围内。一个意外的发现是，这两种DNA都能够转染REP-型莱氏无胆甾原体菌株，该变体由于病毒DNA复制受阻，是单链DNA支原体病毒的非允许宿主（诺瓦克等人，《细菌学杂志》127:832 - 836, 1976）。转染的REP-细胞产生的病毒数量与转染和感染的野生型细胞产生的病毒数量相当。因此，转染的L51 DNA能够绕过REP-细胞中当这些细胞被L51病毒粒子感染时出现的复制障碍。
