Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Department of Biology, Faculty of Science, Islamic Azad University, Science and Research Branch, Tehran, Iran.
Mol Cell Probes. 2023 Aug;70:101916. doi: 10.1016/j.mcp.2023.101916. Epub 2023 Jul 1.
Oral squamous cell carcinoma (OSCC) is the most common cancers arising from the head and neck region. There is growing evidence that lncRNAs play an important role in OSCC progression. The study aims to investigate correlations between the expression levels of LncRNAs of PARROT, MYCNUT, DANCR, and KTN1-AS1 with clinicopathological characteristics and finding suitable biomarkers for OSCC.
Total lncRNAs related to cancers and HNSC trascriptomics data were downloaded from lncRNADisease v2.0 database and xenabrowser, respectively. Then, ACO was perfomed on shared of LncRNAs between two databases. Finally, some lncRNAs were proposed as potential biomarkers. Thirty biopsies samples from patients with the OSCC and 30 healthy subjects were collected by the surgery. Questionnaires including clinical and demographic data were filled for all cases. Using Real-time PCR, the expression levels of PARROT, MYCNUT, DANCR, and KTN1-AS1 lncRNAs were quantified.
According to the results,17 novel gene symbol was identified.All the candidate lncRNAs the expression levels of PARROT, MYCNUT, DANCR, and KTN1-AS1 were remarkably upregulated in OSCC tumors in comparison with control group (RQ: 10.00 (P < 0.0001), RQ: 2.920 (P < 0.0001), RQ: 1.623 (P = 0.002), and 4.467 (P < 0.0001), respectively). Also, we found significant associations between tumor lncRNAs expression of PARRPT and DANCER and tumor metastasis (P = 0.009, and P = 0.005, respectively). Additionally, lncRNA KTN1-AS1 expression level was significantly higher in the patients with tumor size more than 3 cm, in comparison with tumor less than 3 cm (P = 0.005). According ROC analysis, all these candidate lncRNAs can be a significant predictor for OSCC (AUC of PARROT lncRNA = 69.72%, AUC of MYCNUT = 98.22%, AUC of DANCR = 74.83%, and AUC of KTN1-AS1 = 99.22%).
we found that overexpression levels of PARROT, MYCNUT, DANCR, and KTN1-AS1 lncRNAs were correlated with poor clinicopathological characteristics in patients with OSCC. Also, PARROT, MYCNUT, DANCR, and KTN1-AS1 are novel biomarker for the detection of OSCC.
口腔鳞状细胞癌（OSCC）是头颈部最常见的癌症。越来越多的证据表明，lncRNAs 在 OSCC 进展中发挥重要作用。本研究旨在探讨 PARROT、MYCNUT、DANCR 和 KTN1-AS1 的 lncRNA 表达水平与临床病理特征的相关性，并寻找适合 OSCC 的生物标志物。
从 lncRNADisease v2.0 数据库和 xenabrowser 分别下载与癌症相关的总 lncRNAs 和 HNSC 转录组学数据。然后，在两个数据库之间共享的 lncRNAs 上进行 ACO。最后，提出了一些 lncRNAs 作为潜在的生物标志物。通过手术从 30 名 OSCC 患者和 30 名健康受试者中采集 30 个活检样本。所有病例均填写临床和人口统计学数据的问卷。使用实时 PCR 定量 PARROT、MYCNUT、DANCR 和 KTN1-AS1 lncRNAs 的表达水平。
根据结果，鉴定出 17 个新的基因符号。与对照组相比，PARROT、MYCNUT、DANCR 和 KTN1-AS1 的所有候选 lncRNA 的表达水平在 OSCC 肿瘤中均显著上调（RQ：10.00（P < 0.0001），RQ：2.920（P < 0.0001），RQ：1.623（P = 0.002），4.467（P < 0.0001））。此外，我们发现 PARRPT 和 DANCER 肿瘤 lncRNAs 表达与肿瘤转移之间存在显著关联（P = 0.009 和 P = 0.005）。此外，与肿瘤小于 3cm 的患者相比，lncRNA KTN1-AS1 的表达水平在肿瘤大于 3cm 的患者中显著升高（P = 0.005）。根据 ROC 分析，所有这些候选 lncRNA 均可作为 OSCC 的显著预测因子（PARROT lncRNA 的 AUC = 69.72%，MYCNUT 的 AUC = 98.22%，DANCR 的 AUC = 74.83%，KTN1-AS1 的 AUC = 99.22%）。
我们发现 PARROT、MYCNUT、DANCR 和 KTN1-AS1 lncRNAs 的高表达水平与 OSCC 患者的不良临床病理特征相关。此外，PARROT、MYCNUT、DANCR 和 KTN1-AS1 是 OSCC 检测的新型生物标志物。
