Department of Biology, Jeju National University, Jeju 63243, South Korea.
Department of Biology, Jeju National University, Jeju 63243, South Korea.
Mar Genomics. 2023 Aug;70:101046. doi: 10.1016/j.margen.2023.101046. Epub 2023 Jun 8.
As a moderate halophilic-heterotrophic bacterium, Halomonas alkaliantarctica MSP3 was isolated from marine sediment located in Jeju island, South Korea. The complete genome of strain MSP3 was sequenced and analyzed to reveal its genetic features and metabolic potential. The genome size of MSP3 was about 4.23 Mbp with 54.7% G + C content, and it contained 3811 protein-coding sequences and 79 RNA genes (61 tRNA and 18 rRNA). According to the genome annotation, it was revealed that the strain MSP3 harbors genes encoding for urease and urea transporters, which play a crucial role in the process of urea degradation and utilization. In addition, it is noteworthy that the MSP3 strain possesses genes encoding for both cytochrome c oxidase and cytochrome bd oxidase, thereby conferring upon it the ability to adapt to various levels of oxygen (oxic to microoxic) and to execute denitrification processes in the absence of oxygen. Moreover, it was observed that strain MSP3 had genes for the glyoxylate cycle, which is an alternative pathway to the TCA cycle. Furthermore, it was observed that the MSP3 strain exhibited the ability to thrive across a diverse spectrum of NaCl concentrations, ranging from 2% to 10% (w/v). Collectively, strain MSP3 may possess an advantage over competitors within the marine ecosystem, particularly in conditions where carbon substrates are restricted. The genomic-based assumption could potentially be substantiated by the presence of a multitude of transporter genes within the genome.
作为一种中度嗜盐异养细菌，极地盐单胞菌 MSP3 是从韩国济州岛海洋沉积物中分离出来的。对 MSP3 菌株的全基因组进行了测序和分析，以揭示其遗传特征和代谢潜力。MSP3 的基因组大小约为 4.23 Mbp，G+C 含量为 54.7%，包含 3811 个蛋白编码序列和 79 个 RNA 基因（61 个 tRNA 和 18 个 rRNA）。根据基因组注释，发现该菌株 MSP3 含有编码脲酶和尿素转运蛋白的基因，这些基因在尿素降解和利用过程中发挥着关键作用。此外，值得注意的是，MSP3 菌株同时拥有编码细胞色素 c 氧化酶和细胞色素 bd 氧化酶的基因，使其能够适应各种氧水平（从需氧到微需氧），并在缺氧条件下进行反硝化过程。此外，还观察到 MSP3 菌株具有乙醛酸循环的基因，这是三羧酸循环的替代途径。此外，还观察到 MSP3 菌株能够在从 2%到 10%（w/v）的不同 NaCl 浓度范围内茁壮成长。总的来说，MSP3 菌株在海洋生态系统中可能比竞争者具有优势，特别是在碳底物受到限制的情况下。基于基因组的假设可能得到基因组中存在大量转运基因的支持。
