Yu Chengchen, Wu Minglin, Jiang Yuchen, Xu Xiaoyan, Li Jiale, Shen Yubang
Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China.
Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, 201306, China.
Mar Biotechnol (NY). 2023 Aug;25(4):557-566. doi: 10.1007/s10126-023-10225-4. Epub 2023 Jun 24.
Grass carp (Ctenopharyngodon idella) is one of the most economically important fish in China, and its production is commonly lost due to GCRV infection. To understand the molecular mechanism of GCRV resistance in grass carp, we compared the spleen transcriptome of the GCRV-resistant and susceptible individuals under GCRV infection (Res-Sus) and the GCRV-resistant individuals under different conditions of injection with GCRV and PBS (Res-Ctl). A total of 87.56 GB of clean data were obtained from 12 transcriptomic libraries of spleen tissues. A total of 379 DEGs (156 upregulated genes and 223 downregulated genes) were identified in the comparison group Res-Ctl. A total of 1207 DEGs (633 upregulated genes and 574 downregulated genes) were identified in the comparison group Res-Sus. And 54 DEGs were shared including immune-related genes of stc2 (stanniocalcin 2), plxna1 (plexin A1), ifnα (interferon alpha), cxcl 11 (C-X-C motif chemokine ligand 11), ngfr (nerve growth factor receptor), mx (MX dynamin-like GTPase), crim1 (cysteine-rich transmembrane BMP regulator 1), plxnb2 (plexin B2), and slit2 (slit guidance ligand 2). KEGG pathway analysis revealed significant differences in the expression of genes mainly involved in immune system and signal transduction, including antigen processing and presentation, Toll-like receptor signaling pathway, natural killer cell-mediated cytotoxicity, and Hippo signaling pathway. This study investigates the immune mechanism of the resistance to GCRV infection in grass carp and provides useful information for the development of methods to control the spread of the GCRV infection.
草鱼（Ctenopharyngodon idella）是中国经济价值最重要的鱼类之一，其产量常因感染草鱼呼肠孤病毒（GCRV）而损失。为了解草鱼对GCRV抗性的分子机制，我们比较了GCRV感染下抗GCRV和易感个体的脾脏转录组（Res-Sus）以及不同条件下注射GCRV和磷酸盐缓冲液（PBS）的抗GCRV个体的脾脏转录组（Res-Ctl）。从12个脾脏组织转录组文库中总共获得了87.56GB的clean数据。在比较组Res-Ctl中总共鉴定出379个差异表达基因（DEG）（156个上调基因和223个下调基因）。在比较组Res-Sus中总共鉴定出1207个DEG（633个上调基因和574个下调基因）。共有54个DEG是共有的，包括与免疫相关的基因，如stc2（鲟钙蛋白2）、plxna1（丛蛋白A1）、ifnα（α干扰素）、cxcl 11（C-X-C基序趋化因子配体11）、ngfr（神经生长因子受体）、mx（MX动力蛋白样GTP酶）、crim1（富含半胱氨酸的跨膜BMP调节因子1）、plxnb2（丛蛋白B2）和slit2（slit引导配体2）。京都基因与基因组百科全书（KEGG）通路分析显示，主要参与免疫系统和信号转导的基因表达存在显著差异，包括抗原加工和呈递、Toll样受体信号通路、自然杀伤细胞介导的细胞毒性和Hippo信号通路。本研究调查了草鱼对GCRV感染抗性的免疫机制，并为开发控制GCRV感染传播的方法提供了有用信息。
