Yang Shu, Chen Zhijie, Zhuang Pengzhen, Tang Yunkai, Chen Zehao, Wang Fei, Cai Zhengwei, Wei Jie, Cui Wenguo
Department of Orthopaedics, Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, P. R. China.
Shanghai Key Laboratory of Advanced Polymeric Materials, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, 200237, P. R. China.
Small Methods. 2023 Oct;7(10):e2300370. doi: 10.1002/smtd.202300370. Epub 2023 Jun 25.
Current artificial designs of the periosteum focus on osteogenic or angiogenic properties, while ignoring the filling and integration with bone microcracks, which trigger a prolonged excessive inflammatory reaction and lead to failure of bone regeneration. In this study, seamless adhesive biomimetic periosteum patches (HABP/Sr-PLA) were prepared to fill microcracks in defective bone via interfacial self-assembly induced by Sr ions mediated metal-ligand interactions among pamidronate disodium-modified hyaluronic acid (HAPD), black phosphorus (BP), and hydrophilic polylactic acid (PLA). In vitro, HABP/Sr-PLA exhibited excellent self-healing properties, seamlessly filled bone microcracks, and significantly enhanced osteogenesis and angiogenesis. Furthermore, in a rat cranial defect model, HABP/Sr-PLA was demonstrated to significantly promote the formation of blood vessels and new bone under mild 808 nm photothermal stimulation (42.8 °C), and the highest protein expression of CD31 and OPN was five times higher than that of the control group and other groups. Therefore, the proposed seamless microcrack-filled bionic periosteum patch is a promising clinical strategy for promoting bone repair.
目前骨膜的人工设计主要关注成骨或血管生成特性，却忽略了与骨微裂纹的填充和整合，这会引发长期过度的炎症反应并导致骨再生失败。在本研究中，通过帕米膦酸二钠修饰的透明质酸（HAPD）、黑磷（BP）和亲水性聚乳酸（PLA）之间由Sr离子介导的金属-配体相互作用诱导的界面自组装，制备了无缝粘合仿生骨膜贴片（HABP/Sr-PLA），以填充缺损骨中的微裂纹。在体外，HABP/Sr-PLA表现出优异的自愈性能，能无缝填充骨微裂纹，并显著增强成骨和血管生成。此外，在大鼠颅骨缺损模型中，HABP/Sr-PLA被证明在温和的808 nm光热刺激（42.8℃）下能显著促进血管和新骨的形成，CD31和OPN的最高蛋白表达比对照组和其他组高五倍。因此，所提出的无缝填充微裂纹的仿生骨膜贴片是促进骨修复的一种有前景的临床策略。
