Wang Ningtao, Chen Jie, Chen Yanyang, Chen Liang, Bao Luhan, Huang Zhengmei, Han Xiaoyu, Lu Jiangkuo, Cai Zhengwei, Cui Wenguo, Huang Zhengwei
Department of Endodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, 639 Zhizaoju Road, Shanghai, 200011, PR China.
Department of Orthopaedics, Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, Shanghai, 200025, PR China.
Bioact Mater. 2024 Jun 21;40:430-444. doi: 10.1016/j.bioactmat.2024.06.021. eCollection 2024 Oct.
Irregular bone defects, characterized by unpredictable size, shape, and depth, pose a major challenge to clinical treatment. Although various bone grafts are available, none can fully meet the repair needs of the defective area. Here, this study fabricates a dough-type hydrogel (DR-Net), in which the first dynamic network is generated by coordination between thiol groups and silver ions, thereby possessing kneadability to adapt to various irregular bone defects. The second rigid covalent network is formed through photocrosslinking, maintaining the osteogenic space under external forces and achieving a better match with the bone regeneration process. , an irregular alveolar bone defect is established in the fresh porcine mandible, and the dough-type hydrogel exhibits outstanding shape adaptability, perfectly matching the morphology of the bone defect. After photocuring, the storage modulus of the hydrogel increases 8.6 times, from 3.7 kPa (before irradiation) to 32 kPa (after irradiation). Furthermore, this hydrogel enables effective loading of P24 peptide, which potently accelerates bone repair in Sprague-Dawley (SD) rats with critical calvarial defects. Overall, the dough-type hydrogel with kneadability, space-maintaining capability, and osteogenic activity exhibits exceptional potential for clinical translation in treating irregular bone defects.
形状不规则的骨缺损，其大小、形状和深度难以预测，给临床治疗带来了重大挑战。尽管有多种骨移植材料可用，但没有一种能完全满足缺损区域的修复需求。在此，本研究制备了一种面团型水凝胶（DR-Net），其中第一个动态网络由硫醇基团与银离子之间的配位作用产生，从而具有可揉捏性以适应各种不规则骨缺损。第二个刚性共价网络通过光交联形成，在外力作用下维持成骨空间，并与骨再生过程实现更好的匹配。在新鲜猪下颌骨中建立不规则牙槽骨缺损，面团型水凝胶表现出出色的形状适应性，与骨缺损形态完美匹配。光固化后，水凝胶的储能模量增加了8.6倍，从3.7 kPa（辐照前）增加到32 kPa（辐照后）。此外，这种水凝胶能够有效负载P24肽，有力地加速了患有严重颅骨缺损的Sprague-Dawley（SD）大鼠的骨修复。总体而言，具有可揉捏性、空间维持能力和成骨活性的面团型水凝胶在治疗不规则骨缺损的临床转化方面展现出非凡的潜力。
