Hong Yi, Liu Kang-Kang, Wang Ning-Ling, Xie Zhi-Wei, Chu Jin-Hua
Department of Pediatrics, The Second Hospital of Anhui Medical University, Hefei 230061, Anhui Province, China.
Department of Pediatrics, The Second Hospital of Anhui Medical University, Hefei 230061, Anhui Province, China,E-mail:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2023 Jun;31(3):643-648. doi: 10.19746/j.cnki.issn.1009-2137.2023.03.004.
To explore the expression level of exosome derived miR-181b-5p in different disease stages of children with acute lymphoblastic leukemia and its relationship with clinical characteristics.
Bone marrow plasma samples of 86 children with ALL were collected. Exosomes were extracted by exosome extraction kit, and RNA in exosomes was extracted by TRIzol method. The levels of miR-181b-5p in the blood plasma exosomes of the patients in the newly diagnosed group, relapse group, remission group and control group were detected by qRT- PCR. The difference of miR-181b-5p expression level in each group was compared and analyzed, and the relationship between miR-181b-5p expression level and clinical characteristics was analyzed.
The expression level of exosomal miR-181b-5p in the newly diagnosed group and the relapsed group was significantly lower than that in the remission group and the control group (< 0.05). The expression level of exosomal miR-181b-5p in T-ALL children was higher than that in B-ALL children (<0.05). The expression level of plasma exosomal miR-181b-5p in male children was higher than that in female children (<0.01).
Exosome derived miR-181b-5p changes dynamically in the course of ALL children, and can be used as a marker miRNA to monitor disease status. Exosomes can transmit information in the tumor microenvironment and serve as a potential carrier for biomolecular targeted therapy.
探讨外泌体来源的miR-181b-5p在急性淋巴细胞白血病患儿不同疾病阶段的表达水平及其与临床特征的关系。
收集86例急性淋巴细胞白血病患儿的骨髓血浆样本。采用外泌体提取试剂盒提取外泌体，用TRIzol法提取外泌体中的RNA。采用qRT-PCR检测初诊组、复发组、缓解组及对照组患儿血浆外泌体中miR-181b-5p的水平。比较分析各组miR-181b-5p表达水平的差异，并分析miR-181b-5p表达水平与临床特征的关系。
初诊组和复发组外泌体miR-181b-5p的表达水平显著低于缓解组和对照组（<0.05）。T-ALL患儿外泌体miR-181b-5p的表达水平高于B-ALL患儿（<0.05）。男性患儿血浆外泌体miR-181b-5p的表达水平高于女性患儿（<0.01）。
外泌体来源的miR-181b-5p在急性淋巴细胞白血病患儿病程中动态变化，可作为监测疾病状态的标志物miRNA。外泌体可在肿瘤微环境中传递信息，作为生物分子靶向治疗的潜在载体。
