Tada T, Kodama T, Watanabe S, Sato Y, Shimosato Y
Jpn J Clin Oncol. 1986 Jun;16(2):129-35. doi: 10.1093/oxfordjournals.jjco.a039128.
In vitro cell kinetic studies of 27 surgically removed human lung cancers were performed by the immunohistochemical procedure using monoclonal antibody to bromodeoxyuridine (BrdU). BrdU was incorporated under hyperbaric oxygenation, and labeled cells were found to be evenly distributed in the tissue blocks. A comparison of the in vitro and in vivo labeling index (L.I.) in human tumors transplanted to nude mice showed good correlation. Surgically resected adenocarcinoma of the human lung had a significantly lower L.I. than squamous cell carcinoma, the mean values being 6.0% and 17.2%, respectively. In adenocarcinoma, L.I. values disclosed a trend toward a higher L.I. in poorly differentiated carcinoma.
采用抗溴脱氧尿苷（BrdU）单克隆抗体免疫组化方法，对27例手术切除的人肺癌进行了体外细胞动力学研究。在高压氧条件下掺入BrdU，发现标记细胞在组织块中均匀分布。移植到裸鼠体内的人肿瘤的体外和体内标记指数（L.I.）比较显示出良好的相关性。手术切除的人肺腺癌的L.I.明显低于鳞状细胞癌，平均值分别为6.0%和17.2%。在腺癌中，L.I.值显示出低分化癌的L.I.有升高趋势。
