Yoshida K, Morinaga S, Shimosato Y, Hayata Y
Pathology Division, National Cancer Center Research Institute, Tokyo, Japan.
Cancer. 1989 Dec 1;64(11):2284-91. doi: 10.1002/1097-0142(19891201)64:11<2284::aid-cncr2820641118>3.0.co;2-y.
By in vitro labeling with bromodeoxyuridine (BrdU) and immunohistochemical staining with anti-BrdU monoclonal antibody, the tumor cell kinetics were investigated in 52 resected pulmonary adenocarcinomas. The values of the BrdU labeling index (LI) varied widely among cases of adenocarcinoma in comparison with four cases of small cell carcinoma and ten cases of squamous cell carcinoma examined as controls. The LI was below 3.0 in 60% of the adenocarcinomas. When the LI was analyzed according to the cytologic subtypes of adenocarcinoma, the values were low for goblet cell type and type II alveolar cell type, whereas the values for the bronchial surface epithelial cell type varied widely (average, 5.2) and those for the Clara cell type were intermediate (average, 1.8). A comparison of the LI with TNM classification, histologic differentiation, degree of nuclear atypia, and mitotic cell count showed a correlation between the LI and each of these prognostic factors. Thus, the possibility of the BrdU LI being a useful new prognostic factor of pulmonary adenocarcinomas was suggested.
通过用溴脱氧尿苷（BrdU）进行体外标记以及用抗BrdU单克隆抗体进行免疫组织化学染色，对52例切除的肺腺癌的肿瘤细胞动力学进行了研究。与作为对照检查的4例小细胞癌和10例鳞状细胞癌相比，腺癌病例中BrdU标记指数（LI）的值差异很大。60%的腺癌中LI低于3.0。当根据腺癌的细胞亚型分析LI时，杯状细胞型和II型肺泡细胞型的值较低，而支气管表面上皮细胞型的值差异很大（平均为5.2），克拉拉细胞型的值处于中间水平（平均为1.8）。将LI与TNM分类、组织学分化、核异型程度和有丝分裂细胞计数进行比较，结果显示LI与这些预后因素中的每一个均存在相关性。因此，提示BrdU LI有可能成为肺腺癌一个有用的新预后因素。
